Reagents for reducing leukocyte interference in immunoassays
AssigneeAbbott Point of Care Inc.
InventorsJohn Lewis Emerson Campbell, Graham Davis, John Emegbero Omakor, Adam Roger Moss, Ganesh Guhados
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Abstract
Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample such as a whole blood sample with one or more leukocidal reagents that reduce or eliminate the metabolic activity of leukocytes, and performing an immunoassay on the amended sample to determine the concentration of analyte in the sample. Preferably, the sample is amended with one or more enzymes and optionally one or more enzyme substrates and cofactors.
Claims38
A method of performing an electrochemical immunoassay for a target analyte in a whole blood sample comprising:
- contacting the whole blood sample with an oxygen depleting leukocidal reagent, wherein said leukocidal reagent substantially inhibits phagocytic activity of leukocytes in the whole blood sample;
- and
- performing the electrochemical immunoassay on the whole blood sample at an immunosensor to detect a target analyte.
A kit for performing an electrochemical immunoassay for a target analyte in a whole blood sample comprising:
- an immunosensor;
- an oxygen depleting leukocidal reagent, wherein said leukocidal reagent is configured to substantially inhibit phagocytic activity of leukocytes in the whole blood sample, and
- immunoassay reagents for detecting the target analyte.
Dependencies are inferred from the claim text.