US 8,476,054 B2 · Thrombin-like enzyme of Agkistrodon acutus

Abstract

A thrombin-like enzyme isolated from Agkistrodon acutus venom, comprising an alpha subunit having the sequence of SEQ ID No. 1 and a beta subunit having the sequence of SEQ ID No. 2, which are linked by seven disulfide bonds, is provided. The hemocoagulase of Agkistrodon acutus in the present invention is a serine proteinase having a molecular weight of 29.3-29.5 kD and an isoelectric point of 5.5, and is able to hydrolyze the alpha chain of human fibrinogen. The invention also provides methods of purifying the thrombin-like enzyme from snake venom, which comprise removing insoluble substance by pretreatment, conducting twice of anion-exchange column chromatography, collecting active eluting peak, dialyzing, ultra-filtrating and desalinating so as to obtain a snake venom thrombin-like enzyme.

Claims11

Dependent claims

A composition comprising an isolated hemocoagulase of Agkistrodon acutus,

the isolated hemocoagulase consisting of an alpha subunit and a beta subunit, which are linked by seven disulfide bonds,
wherein the alpha subunit consists of the 129 amino acids as set forth in SEQ ID No. 1, and the beta subunit consists of the 123 amino acids as set forth in SEQ ID No. 2,
wherein the hemocoagulase has a molecular weight, as determined by sequence analysis, of about 29.3 kD to about 29.5 kD and an isoelectric point of about 5.5,
wherein the isolated hemocoagulase constitutes more than 95% of the composition.

1 more element

Dependencies are inferred from the claim text.

Thrombin-like enzyme of Agkistrodon acutus

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Thrombin-like enzyme of Agkistrodon acutus

Life of this patent

Abstract

Claims11