Method for determining capability of a compound for inhibiting a receptor using codon-optimized nucleic acid
AssigneeJnc Corporation
InventorsSatoshi Inouye, Yuiko Sahara, Junichi Sato
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Abstract
A method for determining a capability of a compound for inhibiting a receptor through a change of intracellular calcium ion when the receptor is activated is provided. Contacting a host cell genetically engineered to express an apo-clytin-II protein generated from a codon-optimized nucleic acid with a test compound, and then determining an amount of light generated. The intracellular calcium ion can be detected much more easily.
Claims5
A method for determining a capability of a compound for inhibiting a receptor through a change of intracellular calcium ion when the receptor is activated, the method comprising:
- providing a host cell comprising the receptor, and the host cell genetically engineered to express an apo-clytin-II protein coded from a codon-optimized nucleic acid, wherein said codon optimized nucleic acid is SEQ ID NO:3;
- contacting the host cell with coelenterazine or a derivative of coelenterazine;
- adding the compound to and incubating with the host cell;
- and
- determining an amount of light generated from the host cell.
Dependencies are inferred from the claim text.