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US 8,476,036 B2Granted
Nonluminogenic assay for living cells
AssigneePromega Corporation
InventorsAndrew Niles, Terry L. Riss
Life of this patent
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Abstract
A method to detect the presence or amount of at least one molecule for an enzyme-mediated reaction in a multiplex luminogenic/nonluminogenic assay is provided.
Claims14
A method to quantify live cells in a sample, comprising:
- a) contacting a sample comprising live cells and dead cells with a fluorogenic substantially live cell permeable substrate for (i) a protease associated with a proteasome, (ii) an aminopeptidase or (iii) a cathepsin;
- and
- b) detecting or determining total fluorescence in the sample comprising live cells and dead cells, wherein the total fluorescence is proportional to the number of live cells, thereby quantifying the number of live cells in the sample;
- wherein the protease associated with the proteasome, the aminopeptidase or the cathepsin is substantially inactive when present in an extracellular environment.
1 more element
A method to quantify live cells in a sample, comprising:
- a) contacting a sample with a fluorogenic substantially live cell permeable substrate for (i) a protease associated with a proteasome, (ii) an aminopeptidase or (iii) a cathepsin;
- and
- b) detecting or determining total fluorescence in the sample, wherein the total fluorescence is proportional to the number of live cells, thereby quantifying the number of live cells in the sample;
- wherein the protease associated with the proteasome, the aminopeptidase or the cathepsin is substantially inactive when present in an extracellular environment.
1 more element
Dependencies are inferred from the claim text.