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US 8,476,032 B2Granted
Complement factor H-based assays for serum bactericidal activity against Neisseria meningitidis
AssigneesChildren's Hospital & Research Center Oakland, Novartis Vaccines and Diagnostics, Inc., University of Massachusetts
InventorsDan M. Granoff, JoAnne Welsch, Sanjay Ram
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Abstract
Assays for detection of bactericidal anti-Neisserial antibodies using a factor H polypeptide having a human amino acid sequence that mediates binding to Neisserial factor H binding protein (fHBp) are provided, as well as non-human animal models of Neisserial infection.
Claims20
A method of detection of bactericidal antibodies specific to Neisseria meningitidis in a biological sample, the method comprising:
- combining in a reaction mixture:
- viable Neisseria meningitidis expressing a surface factor H binding protein (fHbp);
- a biological sample from a human suspected of containing bactericidal antibodies specific to Neisseria meningitidis,
- a substantially purified factor H (fH) polypeptide comprising the amino acid sequence of a human Short Consensus Repeat 6 (SCR 6);
- and
- a complement of a non-human animal having no detectable anti-Neisseria meningitidis bactericidal activity;
- and
- detecting the presence or the absence of the bactericidal antibodies specific to the Neisseria meningitidis in the sample by assessing viability of the Neisseria meningitidis,
- wherein decreased viability of the Neisseria meningitidis in the presence of the sample indicates that the sample contains the bactericidal antibodies specific to the Neisseria meningitidis.
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Dependencies are inferred from the claim text.