Detection of cannabis use
AssigneeTeknologian tutkimuskeskus VTT Oy
InventorsKristiina Takkinen, Hans Söderlund, Timo Pulli
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Abstract
A binding partner, especially an antibody fragment that specifically recognizes an antigen-antibody immune complex between anti-THC and THC (tetrahydrocannabinol), is disclosed. The binding partner facilitates a non-competitive homogenous immunoassay for detection of cannabis use. A test kit comprising the binding partner is also described. Preferably the immunoassay is applied for roadside testing of saliva from suspected drivers.
Claims17
An isolated binding partner comprising the complementary determining regions (CDRs) of an antibody light chain and heavy chain, wherein said light chain regions have the amino acid sequences of amino acids 23-35, 51-57, and 93-100 of SEQ ID NO:3, and said heavy chain regions have the amino acid sequences of amino acids 29-35, 50-60 and 99-109 of SEQ ID NO:4.
An immunoassay for detecting a cannabinoid in a sample taken from a body, comprising:
- reacting the sample with a reagent pair comprising a first binding partner that specifically binds to the cannabinoid to form a complex, and a second binding partner that specifically binds to the complex;
- and
- determining the binding of the second binding partner to the complex, thereby indicating presence of the cannabinoid in the sample,
- wherein said first binding partner comprises the complementary determining regions (CDRs) of an antibody light chain and heavy chain, wherein said light chain regions have the amino acid sequences of amino acids 24-35, 51-57, and 90-98 of SEQ ID NO:1, and said heavy chain regions have the amino acid sequences of amino acids 27-36, 51-66 and 99-107 of SEQ ID NO:2, and
- said second binding partner comprises the complementary determining regions (CDRs) of an antibody light chain and heavy chain, said light chain regions having the amino acid sequences of amino acids 23-35, 51-57, and 93-100 of SEQ ID NO:3, and said heavy chain regions having the amino acid sequences of amino acids 29-35, 50-60 and 99-109 of SEQ ID NO:4.
Dependencies are inferred from the claim text.