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US 8,476,024 B2Granted
Botulinum neurotoxin a protein receptor and uses thereof
AssigneeToxogen GmbH
InventorsStefan Mahrhold, Johannes Wilhelm Bigalke, Andreas Rummel, Thomas Binz
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Abstract
The invention relates to an isolated polypeptide of the luminal domain of synaptic vesicle glycoprotein 2C of Homo sapiens wherein at least 70 percent of the amino acid sequence is identical to the amino acid sequence of the SV2C of Homo sapiens. The polypeptide binds the HC fragment of botulinum neurotoxin A provided that the polypeptide is not the synaptic vesicle glycoprotein 2C of Homo sapiens.
Claims24
An isolated polypeptide, wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO:
- 1, and further wherein the isolated polypeptide binds the HC-fragment of botulinum neurotoxin A (BoNT/A), with the proviso that a polypeptide comprising the amino acid sequence of full-length synaptic vesicle glycoprotein 2C (SV2C) of Homo sapiens is excluded.
A fusion protein comprising:
- (a) the luminal domain of the synaptic vesicle glycoprotein 2C (SV2C) of Homo sapiens, wherein the luminal domain of SV2C has the amino acid sequence of SEQ ID NO:
- 1, and further wherein the luminal domain of SV2C binds the Hc-fragment of botulinum neurotoxin A;
- and
- (b) a heterologous amino acid sequence, wherein the heterologous sequence is an epitope or an affinity peptide,
- with the proviso that the fusion protein does not comprise the full-length amino acid sequence of synaptic vesicle glycoprotein 2C (SV2C) of Homo sapiens.
2 more elements
Dependencies are inferred from the claim text.