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AssigneesPioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company
InventorsRobert F. Cressman, Jr., Nancy L. Henderson, David Hondred, Nancy J. Leysens, Zhongsen Li, Natalie N. Weber, Aiqui Xing, Cathy Ziaoyan Zhong
Estimating the term…
Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant soybean plants are provided. Specifically, soybean plants having a 3560.4.3.5 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide are provided. The soybean plant harboring the 3560.4.3.5 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:10 and/or 11. The characterization of the genomic insertion site of the 3560.4.3.5 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean 3560.4.3.5 events are provided.
An isolated polynucleotide comprising SEQ ID NO:
A kit for identifying event 3560.4.3.5 in a biological sample, said kit comprising a first and a second primer, wherein said first and said second primer amplify a polynucleotide comprising a 3560.4.3.5 specific region, wherein said 3560.4.3.5 specific region comprises SEQ ID NO:10.
A DNA detection kit comprising at least one polynucleotide that can specifically detect a 3560.4.3.5 specific region comprising SEQ ID NO:10, wherein said polynucleotide comprises at least one DNA molecule of a sufficient length of contiguous nucleotides identical or complementary to SEQ ID NO:
A method for identifying event 3560.4.3.5 in a biological sample, comprising
A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule, wherein said first DNA molecule comprising a first fragment of SEQ ID NO:
Dependencies are inferred from the claim text.