Microvesicle-based compositions and methods
AssigneeProxy Life Sciene Holdings, Inc.
InventorZbigniew Pietrzkowski
Life of this patent
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Abstract
Methods and compositions for diagnosis and/or analysis of a condition in a mammal are disclosed in which RNA from microvesicles is enriched and differentiated to so obtain a result that is indicative of the condition of tissue or organ from which the microvesicle originated. In especially preferred embodiments, the condition is a neoplastic disease of a human and can be identified and staged by differential analysis of one or more distinct RNAs, optionally together with identification and analysis of a non-RNA component of the microvesicle.
Claims13
A method of isolating and characterizing a first mammalian cellular RNA of a plurality of extracellular microvesicles, the method comprising the steps of:
- providing a sample that includes a plurality of mammalian cells and a plurality of extracellular microvesicles;
- separating the plurality of microvesicles from the plurality of mammalian cells, and detecting the first mammalian cellular RNA from the separated extracellular microvesicles using a step of hybridization or amplification, wherein the detected first mammalian cellular RNA is a known marker of a disease;
- detecting a second mammalian cellular RNA from the sample using a second step of hybridization or amplification;
- and
- correlating the detected first mammalian cellular RNA with the detected second mammalian cellular RNA to thereby characterize the first mammalian cellular RNA.
A method of isolating and characterizing a first mammalian cellular RNA of a plurality of extracellular microvesicles, the method comprising the steps of:
- obtaining from a mammal a sample that comprises a plurality of extracellular microvesicles that include the first mammalian cellular RNA, wherein the sample further comprises a second mammalian cellular RNA;
- detecting the first mammalian cellular RNA from the plurality of extracellular microvesicles using a step of hybridization or amplification, wherein the detected RNA is a known marker of a disease;
- detecting the second mammalian cellular RNA from the sample using a second step of hybridization or amplification;
- and
- correlating the detected first mammalian cellular RNA with the detected second mammalian cellular RNA to thereby characterize the first mammalian cellular RNA.
In a method of isolating a mammalian cellular RNA from a biological fluid, the improvement comprising:
- obtaining from a mammal a biological fluid, wherein the biological fluid comprises a plurality of extracellular microvesicles;
- isolating the plurality of extracellular microvesicles from the biological fluid;
- and
- detecting the mammalian cellular RNA from the plurality of extracellular microvesicles using a step of hybridization or amplification, wherein the detected RNA is a known marker of a disease.
Dependencies are inferred from the claim text.