Back to results
US 8,476,006 B2Granted
Store-operated calcium cellular assay
AssigneeNational Center for Biological Sciences
InventorsGayatri Venkiteswaran, Gaiti Hasan
Life of this patent
Estimating the term…
Abstract
The present technology provides a cell based assay for identifying compounds that modulate store-operated ionic calcium levels using itpr mutant cell lines, such as itpr-ku cells, which have abnormal ionic calcium levels.
Claims8
A method for identifying a compound that modulates store-operated calcium entry (SOCE) levels in a cell, comprising:
- contacting a mutant mammalian cell with a test compound,
- wherein the mutant mammalian cell comprises a residual SOCE level and expresses a single 1,4,5-inositol triphosphate receptor (InsP3R) gene (itpr) product, wherein the itpr gene is an itprka1091/ug3 mutant, and wherein the itprka1091/ug3 product contains G1891S and S224F point mutations;
- and measuring a SOCE level in the test compound contacted cell,
- wherein a measured increase or decrease of the SOCE level versus the residual SOCE level identifies the compound as a compound that modulates SOCE levels.
1 more element
Dependencies are inferred from the claim text.