Methods for producing surfaces that resist non-specific protein binding and cell attachment
AssigneeCorning Incorporated
InventorsXiaoxi (Kevin) Chen, William Galbraith
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Abstract
A method is disclosed herein for treating a polymeric surface to resist non-specific binding of biomolecules and attachment of cells. The method includes the steps of: imparting a charge to the polymeric surface to produce a charged surface; exposing the charged surface to a nitrogen-rich polymer to form a polymerized surface; exposing the polymerized surface to an oxidized polysaccharide to form an aldehyde surface; and exposing the aldehyde surface to a reducing agent. Advantageously, a method is provided which produces surfaces that resist non-specific protein binding and cell attachment and that avoids the use of photochemical reactions or prior art specially designed compounds.
Claims17
A method for treating a polymeric surface to resist non-specific binding of biomolecules and attachment of cells, the method comprising the steps of:
- a) imparting a positive charge to the polymeric surface to produce a positively charged surface;
- b) exposing said positively charged surface to a linker to produce an active surface;
- c) exposing the active surface to a nitrogen-rich polymer to form a polymerized surface;
- d) exposing the polymerized surface to an aldehyde-bearing polymer to form an aldehyde surface;
- and
- e) exposing the aldehyde surface to a reducing agent;
- wherein the linker is selected from the group consisting of dialdehydes, diesters, diimidoesters, NHS-esters, hydrazides, carbodiimides, aryl azides, and heterobifunctional linkers having an ester on one end and an aldehyde on the other end.
Dependencies are inferred from the claim text.