Avian influenza virus live attenuated vaccine and uses thereof
AssigneeUniversity of Maryland, College Park
InventorDaniel R. Perez
Life of this patent
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Abstract
Described in this application are attenuated strains of avian influenza virus containing temperature sensitive mutations in addition to a genetic tag in the PB1 gene. The attenuated viruses are useful as avian and mammalian vaccine for protective immunity against homologous and heterologous lethal challenges with influenza virus. A genetically modified avian influenza virus backbone is described which can be used as a master donor strain for the generation of live attenuated vaccines for epidemic and pandemic influenza.
Claims28
A live attenuated avian influenza virus comprising a PB2 gene with a substitution at position 265, and a PB1 gene with substitutions at positions 391, 581, and 661, said PB1 gene also comprising a genetic tag wherein said genetic tag in PB1 comprises 8 amino acids derived from the influenza virus H3 HA protein sequence, said genetic tag identified as SEQ ID NO:1.
A recombinant attenuated influenza virus, comprising
- (i) an avian influenza virus master backbone comprising internal genes of influenza comprising PB2, PB1, PA, NP, M, and NS segments wherein
- said PB2 gene has a substitution at position 265,
- said PB1 has one or more substitutions at 391, 581, and 661, and a genetic tag, wherein said genetic tag comprises 8 amino acids derived from the influenza virus H3 HA protein sequence said genetic tag identified as SEQ ID NO:1;
- and
- (ii) HA and NA genes from another selected influenza virus.
Dependencies are inferred from the claim text.