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US 8,475,806 B2Granted
High titer recombinant influenza viruses for vaccines and gene therapy
AssigneeWisconsin Alumni Research Foundation
InventorYoshihiro Kawaoka
Life of this patent
Estimating the term…
Abstract
The invention provides a composition useful to prepare high titer influenza viruses, e.g., in the absence of helper virus, which includes a sequence from a high titer influenza virus isolate.
Claims32
A plurality of influenza virus vectors for high titer reassortant recombinant influenza virus production comprising:
- a plurality of vectors for vRNA production comprising:
- a vector comprising an influenza virus PA cDNA having SEQ ID NO:
- 1;
- a vector comprising an influenza virus PB1 cDNA having SEQ ID NO:2;
- a vector comprising an influenza virus PB2 cDNA having SEQ ID NO:3;
- a vector comprising an influenza virus NP cDNA having SEQ ID NO:4;
- a vector comprising an influenza virus M cDNA having SEQ ID NO:5;
- a vector comprising an influenza virus NS cDNA having SEQ ID NO:6;
- a vector comprising an influenza virus H5 HA cDNA;
- and
- a vector comprising an influenza virus NA cDNA that does not include SEQ ID NO:8,
- wherein each vector for vRNA production further comprises a RNA polymerase I promoter and a RNA polymerase I terminator operably linked to the influenza virus cDNA, and wherein the sequences in the vectors for vRNA production are those for a reassortant influenza virus;
- and
- a plurality of vectors for mRNA production comprising:
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PA;
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB1;
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB2, and
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus NP.
15 more elements
A plurality of influenza virus for high titer reassortant recombinant influenza virus production comprising:
- a plurality of vectors for vRNA production comprising:
- a vector comprising an influenza virus PA cDNA including sequences that encode a polypeptide encoded by SEQ ID NO:
- 1;
- a vector comprising an influenza virus PB1 cDNA including sequences that encode a polypeptide encoded by SEQ ID NO:2;
- a vector comprising an influenza virus PB2 cDNA including sequences that encode a polypeptide encoded by SEQ ID NO:3;
- a vector comprising an influenza virus NP cDNA including sequences that encode a polypeptide encoded by SEQ ID NO:4;
- a vector comprising an influenza virus M cDNA including sequences that encode a polypeptide encoded by SEQ ID NO:5;
- a vector comprising an influenza virus NS cDNA including sequences that encode a polypeptide encoded by SEQ ID NO:6;
- a vector comprising an influenza virus H5 HA cDNA;
- and
- a vector comprising an influenza virus NA cDNA that does not include sequences for a polypeptide encoded by SEQ ID NO:8,
- wherein each vector for vRNA production further comprises a RNA polymerase I promoter and a RNA polymerase I terminator operably linked to the influenza virus cDNA, and wherein the sequences in the vectors for vRNA production are those for a reassortant influenza virus;
- and
- a plurality of vectors for mRNA production comprising:
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PA;
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB1;
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB2, and
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus NP.
15 more elements
A plurality of influenza virus vectors for high titer reassortant recombinant influenza virus production comprising:
- a plurality of vectors for vRNA production comprising:
- a vector comprising an influenza virus PA cDNA having SEQ ID NO:
- 1;
- a vector comprising an influenza virus PB1 cDNA having SEQ ID NO:2;
- a vector comprising an influenza virus PB2 cDNA having SEQ ID NO:3;
- a vector comprising an influenza virus NP cDNA having SEQ ID NO:4;
- a vector comprising an influenza virus M cDNA having SEQ ID NO:5;
- a vector comprising an influenza virus NS cDNA having SEQ ID NO:6;
- a vector comprising an influenza virus NA cDNA that does not include sequences for a polypeptide encoded by SEQ ID NO:7;
- and
- a vector comprising an influenza virus NA cDNA that does not include sequences for a polypeptide encoded by SEQ ID NO:8,
- wherein each vector for vRNA production further comprises a RNA polymerase I promoter and a RNA polymerase I terminator operably linked to the influenza virus cDNA, and wherein the sequences in the vectors for vRNA production are those for a reassortant influenza virus;
- and
- a plurality of vectors for mRNA production comprising:
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PA;
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB1;
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB2, and
- a vector comprising a promoter operably linked to a DNA segment encoding influenza virus NP.
15 more elements
Dependencies are inferred from the claim text.