Assays for diagnosis of tuberculosis and uses thereof
AssigneeFusion Antibodies Limited
InventorsShane A. Olwill, Richard J. Buick, Hangfai Kwok, James A. Johnston
Life of this patent
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Abstract
The invention relates to the identification of mycobacterial antigens which are highly immunogenic and which may be used in assays and methods for the diagnosis of tuberculosis and the discrimination between infected animals and animals previously exposed to vaccines.
Claims20
A diagnostic kit for the diagnosis of the presence of tuberculosis in a biological sample, said kit comprising:
- (i) the polypeptide having the amino acid sequence shown as SEQ ID NO:5;
- and
- (ii) the polypeptide having the amino acid sequence shown as SEQ ID NO:1;
- and
- (iii) the polypeptide having the amino acid sequence shown as SEQ ID NO:7;
- and
- (iv) optionally at least one polypeptide selected from the polypeptides having the amino acid sequence shown as any one of SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4;
- and
- (v) optionally, at least one polypeptide selected from the polypeptides having the amino acid sequence shown as any one of SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:23.
A diagnostic kit for the diagnosis of the presence of tuberculosis in a biological sample, wherein the kit comprises:
- a polypeptide having the amino acid sequence shown as SEQ ID NO:5;
- and
- (ii) at least one of the polypeptides having the amino acid sequence shown as SEQ ID NOS:
- 1, 6, 7, 8 or 23;
- and
- (iii) optionally at least one of the polypeptides having the amino acid sequence shown as SEQ ID NO:
- 2, 3 or 4.
A diagnostic it for the diagnosis of the presence of tuberculosis in a biological sample, said kit comprising:
- (i) the polypeptide having the amino acid sequence shown as SEQ ID NO:5;
- and
- (ii) one or more polypeptides selected from the polypeptides having the amino acid sequence shown as any one of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:
- 6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:
- 23.
A method of detecting exposure to Mycobacterium in an animal or in a biological sample from said animal, the method comprising the step of detecting the presence or absence of an immune response to:
- (i) the polypeptide having the amino acid sequence shown as SEQ ID NO:5;
- and
- (ii) the polypeptide having the amino acid sequence shown as SEQ ID NO:1;
- and
- (iii) the polypeptide having the amino acid sequence shown as SEQ ID NO:7;
- and
- (iv) optionally at least one polypeptide selected from the polypeptides having the amino acid sequence shown as any one of SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4;
- and
- (v) optionally at least one polypeptide selected from the polypeptides having the amino acid sequence shown as any one of SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:23;
- wherein the presence of an immune response to one or more of said polypeptides is indicative of the exposure of the animal to Mycobacterium.
An assay method for the detection of the presence of Mycobacterium in an animal or a biological sample from said animal, said method comprising the steps:
- providing a biological sample from said animal;
- and detecting the presence or absence of an immune response to:
- (i) the polypeptide having the amino acid sequence shown as SEQ ID NO:5;
- and
- (ii) one or more polypeptides selected from the polypeptides having the amino acid sequence shown as any one of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:
- 6, SEQ ID NO:7, SEQ ID NO:
- 8, or SEQ ID NO:23;
- in the biological sample;
- wherein the presence of an immune response to one or more of said polypeptides is indicative of the presence of Mycobacterium in the biological sample.
Dependencies are inferred from the claim text.