Methods of treating spinal cord injury and minimizing scarring
AssigneeStemnion, Inc.
InventorsGeorge L. Sing, Diana L. Clarke, Vivienne S. Marshall
Life of this patent
Estimating the term…
Abstract
The invention is directed to methods of promoting the healing of spinal cord injury. The invention is further directed to methods of minimizing the extent of scarring following spinal cord injury. Such methods utilize novel compositions, including but not limited to extraembryonic cytokine secreting cells (herein referred to as ECS cells), including, but not limited to, amnion-derived multipotent progenitor cells (herein referred to as AMP cells) and conditioned media derived therefrom (herein referred to as amnion-derived cellular cytokine solution or ACCS), each alone or in combination with each other and/or other agents.
Claims9
A method for relieving or ameliorating spinal cord injury in a patient in need thereof comprising administering to the patient a therapeutically effective amount of a composition comprising amnion-derived multipotent progenitor cell conditioned media, wherein the conditioned media is made by culturing the amnion-derived multipotent progenitor cells in basal culture medium that is supplemented with human serum albumin and, optionally, further supplemented with recombinant human protein factors capable of stimulating proliferation of the cultured amnion-derived multipotent progenitor cells, and collecting the conditioned medium.
A method for promoting the healing of a spinal cord injury in a patient in need thereof comprising administering to the patient a therapeutically effective amount of a composition comprising amnion-derived multipotent progenitor cell conditioned media, wherein the conditioned media is made by culturing the amnion-derived multipotent progenitor cells in basal culture medium that is supplemented with human serum albumin and, optionally, further supplemented with recombinant human protein factors capable of stimulating proliferation of the cultured amnion-derived multipotent progenitor cells, and collecting the conditioned medium.
A method for stimulating growth or regeneration of spinal cord cells following spinal cord injury in a patient in need thereof comprising administering to the patient a therapeutically effective amount of a composition comprising amnion-derived multipotent progenitor cell conditioned media wherein the conditioned media is made by culturing the amnion-derived multipotent progenitor cells in basal culture medium that is supplemented with human serum albumin and, optionally, further supplemented with recombinant human protein factors capable of stimulating proliferation of the cultured amnion-derived multipotent progenitor cells, and collecting the conditioned medium, and wherein the spinal cord cells are selected from the group consisting of neurons, oligodendrocytes, astrocytes, microglial cells, and ependymal cells.
A method for ameliorating scarring following spinal cord injury in a patient in need thereof comprising administering to the patient a therapeutically effective amount of a composition comprising amnion-derived multipotent progenitor cell conditioned media, wherein the conditioned media is made by culturing the amnion-derived multipotent progenitor cells in basal culture medium that is supplemented with human serum albumin and, optionally, further supplemented with recombinant human protein factors capable of stimulating proliferation of the cultured amnion-derived multipotent progenitor cells, and collecting the conditioned medium.
A method for neuroprotecting spinal cord neurons following spinal cord injury in a patient in need thereof comprising administering to the patient a therapeutically effective amount of a composition comprising amnion-derived multipotent progenitor cell conditioned media, wherein the conditioned media is made by culturing the amnion-derived multipotent progenitor cells in basal culture medium that is supplemented with human serum albumin and, optionally, further supplemented with recombinant human protein factors capable of stimulating proliferation of the cultured amnion-derived multipotent progenitor cells, and collecting the conditioned medium.
Dependencies are inferred from the claim text.