Method for multiplexed capillary electrophoresis signal cross-talk correction

A method of correcting multiplexed capillary electrophoresis (CE) electropherograms for cross-talk, comprising:

• a) preparing an observed signal electropherogram(n) for a selected capillary of a multi-capillary electrophoresis system;
• b) determining if a capillary exists z capillaries away from said selected capillary, and if so,
• i) preparing an observed signal (CE) electropherogram(n+z) for a capillary located z capillaries away from said selected capillary (n)
• ii) selecting a value that is greater than zero or less than or equal to a preset C % value that is less than or equal to 100 and designating this as A % value
• iii) multiplying the A %-value onto each point of said electropherogram(n+z) to result in a modified signal electropherogram (n+z)-mod;
• iv) subtracting said modified signal electropherogram(n+z)-mod from said signal electropherogram(n), resulting in a modified signal electropherogram(n)-mod;
• v) selecting a new A %-value;
• vi) iterating the process steps III)-through v) until the A % value is the largest possible value between zero and said C % value without giving any values in electropherogram(n)-mod less than a predefined B value
• vii) preparing a corrected multiplexed capillary electrophoresis electropherogram by replacing each value of said signal electropherogram(n) with each corresponding value of electropherogram(n)-mod.