# US 8,465,983 B1 · Detection and delivery systems utilizing supported lipid bilayers

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 8,465,983 B1
- Document ID: US8465983B1
- Status: Granted
- Kind: B1
- Application number: 11/466,046
- Filed: 2006-08-21
- Granted: 2013-06-18
- Earliest claimed: 2005-08-19
- Applicants: Gabriel P. Lopez; Reema Zeineldin; Menake E. Piyasena; Sireesha Chemburu
- Inventors: Gabriel P. Lopez; Reema Zeineldin; Menake E. Piyasena; Sireesha Chemburu
- Web page: https://patentgrep.com/patent/US8465983B1

## CPC

- G01N 21/00: Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light (G01N3/00 - G01N19/00 take precedence)
- C12Q 1/18: Testing for antimicrobial activity of a material
- G01N 33/542: with steric inhibition or signal modification, e.g. fluorescent quenching
- G01N 33/54313: {the carrier being characterised by its particulate form}
- G01N 33/5432: {Liposomes or microcapsules}
- G01N 33/554: the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention relates to lipid bilayer coated beads and methods of using those beads in delivery systems, in immunoassays, in analytical assays and the like.

## Claims

### Claim 1

A method of identifying a test agent that disrupts a lipid bilayer, which comprises contacting a test agent with a lipid bilayer-coated bead that has a detectable label encapsulated by the lipid bilayer, and observing whether a signal from the detectable label is altered by a quenching molecule present in solution surrounding the bead when the lipid bilayer coated bead is exposed to the test agent.

### Claim 2 (depends on 1)

The method of claim 1 wherein the bead is porous.

### Claim 3 (depends on 2)

The method of claim 2 wherein the detectable label is present with in the pores of the porous bead.

### Claim 4 (depends on 2)

The method of claim 2 wherein a detectable signal is emitted by the detectable label prior to addition of the test agent.

### Claim 5 (depends on 4)

The method of claim 4 wherein the signal is reduced when the test agent disrupts the lipid bilayer.

### Claim 6 (depends on 5)

The method of claim 5 further comprising detecting disruption of the lipid bilayer with flow cytometry.

### Claim 7 (depends on 2)

The method of claim 2 wherein the detectable label is a fluorescent polymer.

### Claim 8 (depends on 2)

The method of claim 2 wherein the detectable label is selected from the group consisting of: a fluorescent dye, an ion-sensitive dye, a pH-sensitive dye, an enzyme, a chemiluminescent molecule, a chromophore, an enzyme substrate, an enzyme cofactor, or an enzyme inhibitor.

### Claim 9 (depends on 2)

The method of claim 2 wherein the test agent comprises a molecule, a drug, a detergent, a toxin, a polypeptide, a peptide, an antigen, an antibody, an enzyme, a receptor, a ligand, a nucleic acid, a virus, a liposome, a lipid, a surfactant, a toxin, or a combination thereof.

### Claim 10 (depends on 2)

The method of claim 2 wherein the lipid bilayer further comprises a carbohydrate, protein, or combination thereof.

### Claim 11 (depends on 10)

The method of claim 10 wherein the lipid bilayer further comprises a protein and wherein the protein is selected from the group consisting of: a cell membrane protein, antibody, immunoreceptor, or a cellular receptor.

### Claim 12 (depends on 2)

The method of claim 2 wherein a linker is attached to the bead.

### Claim 13 (depends on 12)

The method of claim 12 wherein the linker comprises an alkylene chain, a peptide, a glycan, or a lipid.

### Claim 14 (depends on 12)

The method of claim 12 wherein the linker comprises biotin or streptavidin.

### Claim 15 (depends on 12)

The method of claim 12 wherein the linker is also attached to an antigen or antibody.