# US 8,465,978 B2 · Method for conducting platelete aggregation analysis by a cartridge device > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,465,978 B2 - Document ID: US8465978B2 - Status: Granted - Kind: B2 - Application number: 13/029,575 - Filed: 2011-02-17 - Published: 2011-06-09 - Granted: 2013-06-18 - Earliest claimed: 2011-02-17 - Assignees: F. Hoffmann-La Roche AG - Applicants: Andreas Calatzis; Ben Krüger; Marc Wittwer - Inventors: Andreas Calatzis; Ben Krüger; Marc Wittwer - Web page: https://patentgrep.com/patent/US8465978B2 ## CPC - B01L 3/502: {with fluid transport, e.g. in multi-compartment structures} - G01N 33/86: involving blood coagulating time {or factors, or their receptors} - G01N 27/07: Construction of measuring vessels; Electrodes therefor - G01N 33/4905: {Determining clotting time of blood (by chemical methods G01N33/86, C12Q1/56)} - B01L 2200/028: Modular arrangements - B01L 2300/046: Function or devices integrated in the closure - B01L 2300/0645: Electrodes - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Methods and devices for conducting platelet aggregation analysis. A method for conducting platelet aggregation analysis by a cartridge device, including providing a blood sample in the cartridge device, stirring the blood sample within the cartridge device, measuring the electrical impedance between electrodes to obtain measured electrical impedance values, comparing measured electrical impedance values, discarding and repeating the measurements of the electrical impedance in case a variation of the measurements is outside a predetermined threshold range, or reporting the measured electrical impedance values in case the variation of the measurements is within the predetermined threshold range. Reported measured electrical impedance values indicate platelet aggregation in a blood sample. ## Claims ### Claim 1 A method for conducting platelet aggregation analysis by a cartridge device comprising two, three, or four pairs of electrode wires for measuring the electrical impedance between two respective electrodes of each pair of electrode wires, the method comprising the following steps: (a) providing a blood sample in a cup portion of the cartridge device; (b) stirring said blood sample for circulating said blood sample within said cup portion of the cartridge device; (c) providing an electrode holder having incorporated the two, three, or four pairs of electrode wires, wherein sensor portions of the two, three, or four pairs of electrode wires are completely immersed into the blood sample, wherein the two respective electrodes of the two, three, or four pairs of electrode wires are positioned parallel to each other and parallel to the respective electrodes of the other pairs of electrode wires; (d) measuring the electrical impedance between the respective electrodes of the at least two, three, or four pairs of electrode wires to obtain measured electrical impedance values; (e) comparing the measured electrical impedance values from the two, three, or four pairs of electrode wires immersed in the blood sample to one another; (f) discarding and repeating the measurements of the electrical impedance in case a variation of the measurements is outside a predetermined threshold range; or (g) reporting the measured electrical impedance values from the two, three, or four pairs of electrode wires and/or the mean or median value thereof in case the variation of the measurements is within the predetermined threshold range, wherein the reported measured electrical impedance values indicate platelet aggregation in the blood sample. ### Claim 2 (depends on 1) The method according to claim 1, wherein only those measurement values are rejected, which are outside the predetermined threshold range, wherein the remaining measurement values and/or the mean values thereof are reported. ### Claim 3 (depends on 1) The method of claim 1, wherein at least two separate measurements of impedance are performed in parallel. ### Claim 4 (depends on 3) The method of claim 3, wherein if three pairs of electrode wires are used, the impedance between a first pair of electrode wires and a second pair of electrode wires is measured as a first impedance signal, the impedance between the second pair of electrode wires and a third pair of electrode wires is measured as a second impedance signal, and the impedance between the third pair of electrode wires and the first pair of electrode wires is measured as a third impedance signal. ### Claim 5 (depends on 4) The method of claim 4, wherein if a variation of a determined impedance signal is higher than a predetermined threshold range of a mean value of the first, second and third impedance signals the signal is rejected, and only the remaining two impedance signals are analysed. ### Claim 6 (depends on 5) The method of claim 5, wherein if the impedance of the remaining two impedance signals shows a variation being higher than the predetermined threshold range, the whole analysis is discarded. ### Claim 7 (depends on 5) The method of claim 5, wherein if a variation of a determined impedance signal is lower than the predetermined threshold range of a mean value of the first, second and third impedance signals a mean value of the measured electrical impedance values is reported to a user. ### Claim 8 (depends on 1) The method of claim 1, further comprising adding reagents that either activate or inhibit platelet function to the cup portion of the cartridge device. ### Claim 9 (depends on 1) The method of claim 1, further comprising continuously recording an impedance change on each electrode pair of said two, three or four pairs of electrode wires. ### Claim 10 (depends on 9) The method of claim 9, wherein if an impedance variation is detected between the two electrodes of said two, three or four pairs of electrode wires, said measurement is discarded. ### Claim 11 (depends on 10) The method of claim 10, wherein an automatic algorithm in a software of an analyser connected to the cartridge device distinguishes between an acceptable and a non-acceptable impedance variation and automatically stops the analysis. ### Claim 12 (depends on 1) The method of claim 1, wherein the method provides a continuous signal regarding the kinetics of platelet aggregation. ### Claim 13 (depends on 1) The method of claim 1, wherein spacing between said wires comprised by the pairs of electrode wires is of about 0.5 to 1 mm. ### Claim 14 (depends on 1) The method of claim 1, wherein the wires comprised by said two, three or four pairs of electrode wires consist of a metal having a low tendency to oxidize. ### Claim 15 (depends on 14) The method of claim 14, wherein said metal comprises a first material comprising copper and copper alloy, which is covered by a second material comprising at least one of silver, platinum, and gold. ### Claim 16 (depends on 15) The method of claim 15, wherein the first material is a copper-silver alloy. ### Claim 17 (depends on 16) The method of claim 16, wherein the copper-silver alloy comprises 0.2% to 2% silver. ### Claim 18 (depends on 16) The method of claim 16, wherein the copper-silver alloy comprises 0.9% silver. ### Claim 19 (depends on 15) The method of claim 15, wherein the second material is a silver coating with a mass coverage in the range of 0.5 to 20 g/kg. ### Claim 20 (depends on 15) The method of claim 15, wherein the second material is a silver coating with a mass coverage of about 2 g/kg. ### Claim 21 (depends on 1) The method of claim 1, wherein said cartridge device comprises three electrode wires for measuring the electrical impedance between at least two of the at least three electrode wires.