# US 8,465,976 B2 · Methods for isolating and using hematopoietic and embryonic stem cells of the peritoneal cavity

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- Number: US 8,465,976 B2
- Document ID: US8465976B2
- Status: Granted
- Kind: B2
- Application number: 12/526,661
- Filed: 2008-02-12
- Published: 2010-02-18
- Granted: 2013-06-18
- Earliest claimed: 2007-02-12
- Assignees: Cedars-Sinai Medical Center
- Applicants: Behrooz Sharifi; Lai Wang; Prediman K. Shah
- Inventors: Behrooz Sharifi; Lai Wang; Prediman K. Shah
- Web page: https://patentgrep.com/patent/US8465976B2

## CPC

- C12N 5/0647: {Haematopoietic stem cells; Uncommitted or multipotent progenitors}
- A61L 27/3804: {characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

The invention relates to the isolation and use of hematopoietic and embryonic stem cells. Additionally, the inventors identified the peritoneal cavity as a new source of hematopoietic stem cells. In one embodiment, the invention provides methods of isolating progenitor and/or stem cells from the peritoneal cavity. In another embodiment, the invention provides methods of transporting progenitor and/or stem cells from the peritoneal cavity to another organ. In another embodiment, the present invention provides methods of regenerating bioengineered tissues and/or reconstituting an hematopoietic system.

## Claims

### Claim 1

A method of generating a capsule graft, comprising:
implanting a mechanical substrate in a peritoneum of a mammal; and
harvesting the capsule graft from said mechanical substrate, wherein the capsule graft comprises hematopoietic stem cells, and wherein said harvesting occurs at least 8 weeks after implanting said mechanical substrate in the peritoneum.

### Claim 2 (depends on 1)

The method of claim 1, wherein the hematopoietic stem cells are Lin−/Sca-1+/c-kit+ hematopoietic stem cells.

### Claim 3 (depends on 1)

The method of claim 1, wherein the hematopoietic stem cells stimulate neovascularization when grafted to an organ, vessel or tissue.

### Claim 4

A method of isolating a population of long-term repopulating hematopoietic stem cells, comprising:
implanting a mechanical substrate in a peritoneum of a mammal;
harvesting cells from said mechanical substrate; and
purifying a population of Lin−/Sca-1+/c-kit+ hematopoietic stem cells.

### Claim 5 (depends on 4)

The method of claim 4, wherein the hematopoietic stem cells stimulate neovascularization when grafted to an organ, vessel or tissue.

### Claim 6 (depends on 5)

The method of claim 5, wherein said organ, vessel or tissue is an aorta.

### Claim 7 (depends on 4)

The method of claim 4, wherein mammal is a mouse.

### Claim 8 (depends on 4)

The method of claim 4, wherein mammal is a human.

### Claim 9 (depends on 4)

The method of claim 4, wherein the hematopoietic stem cells are capable of at least partially reconstituting the hematopoietic system of a mammal when grafted to an organ, vessel or tissue.