# US 8,465,972 B2 · Nanoparticulate cell culture surface

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 8,465,972 B2
- Document ID: US8465972B2
- Status: Granted
- Kind: B2
- Application number: 13/555,595
- Filed: 2012-07-23
- Published: 2012-11-15
- Granted: 2013-06-18
- Earliest claimed: 2008-11-26
- Assignees: Corning Incorporated
- Applicants: Wendy A. Baker; Bertrand De Lambert; David Henry; Odessa N. Petzold
- Inventors: Wendy A. Baker; Bertrand De Lambert; David Henry; Odessa N. Petzold
- Web page: https://patentgrep.com/patent/US8465972B2

## CPC

- G01N 33/54353: {with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393)}
- C12M 23/20: {Material Coatings (immunocoatings C12M25/00)}
- C12N 11/082: obtained by reactions only involving carbon-to-carbon unsaturated bonds
- G01N 33/54346: {Nanoparticles}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A cell culture article including a substrate having nanoparticles on the substrate surface, the nanoparticle including: a polymer of formula (I) where (x), (y), (z), R, R′, R″, S, W, and X, are as defined herein. Methods for making the cell culture article or cell culture article and methods for performing an assay of a ligand with the article are also disclosed.

## Claims

### Claim 1

A cell culture article comprising:
a substrate having nanoparticles (NP) on the substrate surface, wherein the nanoparticles comprise:
a polymer of formula (I)
having at least one of: a metal-ion chelating group (x), an ionizable group (y), and a surface substantive group (z),
where
R is hydrogen or a substituted or unsubstituted, linear or branched, monovalent hydrocarbyl moiety having from 1 to 6 carbon atoms;
R′ is a substituted or unsubstituted, linear or branched, divalent hydrocarbyl moiety resulting from copolymerization of an unsaturated monomer having from 2 to 18 carbon atoms with maleic anhydride;
R″ is a substituted or unsubstituted, linear or branched, divalent hydrocarbyl moiety having from 1 to 20 carbon atoms;
S comprises at least one point of attachment to the substrate;
W comprises at least one bi-dentate group;
X is an —NH—, —NR—, or O;
the mole ratio of x:(y+z) groups is from about 2:8 to about 8:2; and the nanoparticles have a diameter of from about 10 to about 100 nanometers.

### Claim 2 (depends on 1)

The article of claim 1 wherein R is a hydroxy substituted, monovalent hydrocarbyl moiety having from 2 to 6 carbon atoms.

### Claim 3 (depends on 1)

The article of claim 1 wherein the mole ratio x:(y+z) is about 1:1.

### Claim 4 (depends on 1)

The article of claim 1 wherein S comprises at least one of:
a metal oxide, a mixed metal oxide, a polymer, a composite, or a combination thereof; a surface modified substrate; or a combination thereof.

### Claim 5 (depends on 1)

The article of claim 1 wherein
R is a hydroxy substituted alkyl having from 2 to 4 carbon atoms;
R′ is a divalent hydrocarbyl moiety having from 2 to 10 carbon atoms;
R″ is a substituted or unsubstituted, divalent hydrocarbyl moiety having from 3 to 6 carbon atoms;
S is an aminosiloxane treated glass or plastic substrate;
W comprises at least one iminodiacetic acid, nitrilotriacetic acid, triazacyclononane, aminoethylethanolamine, triethylenetetramine, 2-hydroxypropane-1,2,3-tricarboxylate, or a mixture thereof;
X is —NH—;
the mole ratio x:(y+z) is from about 2:1 to about 1:2; and
the nanoparticles have a diameter of from about 10 to about 100 nanometers.

### Claim 6 (depends on 1)

The article of claim 1 wherein the nanoparticles (NP) on the substrate surface comprise a layer of nanoparticles, a polymer film, or a combination of nanoparticles and polymer film.

### Claim 7 (depends on 6)

The article of claim 6 wherein the layer or film comprises incomplete substrate surface coverage.

### Claim 8 (depends on 1)

The article of claim 1 wherein
R is a hydroxy substituted alkyl having from 2 to 4 carbon atoms;
R′ is a divalent hydrocarbyl moiety having from 2 to 10 carbon atoms;
R″ is a substituted or unsubstituted, divalent hydrocarbyl moiety having from 3 to 6 carbon atoms;
S is a plastic substrate;
W comprises nitrilotriacetic acid;
X is —NH—;
the mole ratio x:(y+z) is from about 2:1 to about 1:2; and
the nanoparticles have a diameter of from about 10 to about 100 nanometers.