Back to results
US 8,465,965 B2Granted
Reagents and methods for cyanobacterial production of bioplastics and biomaterials
AssigneeArizona Board of Regents
InventorsHatem Mohammed, Wim Vermaas
Life of this patent
Estimating the term…
Abstract
The present invention provides reagents and methods for biomaterial production from cyanobacteria.
Claims7
An isolated recombinant nucleic acid, comprising:
- (a) a first nucleic acid which comprises an inducible cyanobacterial promoter;
- and
- (b) a second nucleic acid operably linked to the first nucleic acid, wherein the second nucleic acid comprises the full-length complement of the polynucleotide of SEQ ID NO:
- 1.
1 more element
An expression vector comprising:
- (a) a first nucleic acid which comprises an inducible cyanobacterial promoter;
- and
- (b) a second nucleic acid operably linked to the first nucleic acid, wherein the second nucleic acid comprises the full-length complement of the polynucleotide of SEQ ID NO:
- 1.
1 more element
A recombinant Synechocystis PCC 6803 host cell, wherein the host cell has been modified to introduce an inactivating insertion or deletion in an endogenous gene encoding the protein of SEQ ID NO:
- 2.
A recombinant Synechocystis PCC 6803 host cell, wherein the host cell comprises a recombinant nucleic acid that comprises:
- (a) a first nucleic acid which comprises an inducible cyanobacterial promoter;
- and
- (b) a second nucleic acid operably linked to the first nucleic acid, wherein the second nucleic acid comprises the full-length complement of the polynucleotide of SEQ ID NO:
- 1.
1 more element
A method for producing a polyhydroxybutyrate (PHB), comprising:
- (a) culturing recombinant Synechocystis PCC 6803 host cells, wherein the host cells have been modified to introduce an inactivating insertion or deletion in an endogenous gene encoding the protein of SEQ ID NO:
- 2;
- (b) harvesting the recombinant Synechocystis PCC 6803 host cells;
- and
- (c) isolating the PHB from the recombinant Synechocystis PCC 6803 host cells.
2 more elements
A method for producing a PHB, comprising:
- (a) culturing recombinant Synechocystis PCC 6803 host cells, wherein the host cells have been transformed with a recombinant nucleic acid, wherein said recombinant nucleic acid comprises:
- (i) a first nucleic acid which comprises an inducible cyanobacterial promoter;
- and
- (ii) a second nucleic acid operably linked to the first nucleic acid, wherein the second nucleic acid comprises the full-length complement of the polynucleotide of SEQ ID NO:
- 1;
- (b) harvesting the recombinant Synechocystis PCC 6803 host cells;
- and
- (c) isolating the PHB from the recombinant Synechocystis PCC 6803 host cells.
5 more elements
Dependencies are inferred from the claim text.