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US 8,465,954 B2Granted
Ethanol production in microorganisms
AssigneeJoule Unlimited Technologies, Inc.
InventorsBrian D. Green, Nikos Basil Reppas, Dan Eric Robertson
Life of this patent
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Abstract
The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.
Claims24
A method for the biogenic production of ethanol, comprising:
- culturing an engineered cyanobacterium in a culture medium in the presence of light and CO2, wherein said cyanobacterium comprises a recombinant pyruvate decarboxylase gene, and a recombinant alcohol dehydrogenase gene encoding an alcohol dehydrogenase which is at least 95% identical to SEQ ID NO:
- 2 having alcohol dehydrogenase activity, and wherein said recombinant pyruvate decarboxylase gene and said recombinant alcohol dehydrogenase gene are differentially expressed.
An engineered cyanobacterium, which comprises a recombinant pyruvate decarboxylase gene, and a recombinant alcohol dehydrogenase gene encoding an alcohol dehydrogenase which is at least 95% identical to SEQ ID NO:
- 2 having alcohol dehydrogenase activity, wherein the activity levels of said recombinant pyruvate decarboxylase gene and said recombinant alcohol dehydrogenase gene can be varied.
Dependencies are inferred from the claim text.