Lantibiotic biosynthetic gene clusters from A. garbadinensis and A. liguriae
AssigneeNovacta Biosystems Limited
InventorsSteven Boakes, Jesus Cortes Bargallo, Michael John Dawson
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Abstract
Characterization of the biosynthetic gene cluster for the lantibiotic antibiotic actagardine, identification of a novel variant of actagardine and its biosynthetic cluster, and methods of production and use of actagardine, a novel actagardine variant, herein referred to as actagardine B, and variants of both of these produced according to this invention, utilizing genes from the characterized biosynthetic gene clusters, is described.
Claims23
A method for producing a host cell suitable for expressing a type B lantibiotic peptide deoxyactagardine, actagardine or a variant thereof, the method comprising the step of
- transforming a cell by introducing:
- a LanA gene, said gene encoding a LanA polypeptide comprising actagardine, actagardine B, actagardine B variant Ll, ala(0)-actagardine, ala(0)-actagardine B, ala(0)-actagardine B variant Ll, the polypeptide having the structure
- wherein
- -X1-X2- is -Leu-Val, -Val-Ile- or -Leu-Ile-;
- Y is —S—or —S(O)—;
- and
- Z is either H2N— or an amino acid,
- or an amino acid variant of any one of the same wherein:
- 1 to 5 amino acids in the LanA polypeptide sequence at positions 0 (Z), 2, 3, 4, 5, 8, 10, 11, 13 and 18 is/are substituted by another amino acid, and
- optionally introducing the LanA gene together with one or more associated cluster genes suitable for conversion of a precursor peptide into said peptide, thereby generating a host cell comprising a polynucleotide sequence encoding said peptide and suitable for expressing same.
Dependencies are inferred from the claim text.