# US 8,465,940 B2 · Method for electrochemically measuring 1,5-anhydroglucitol in whole blood

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- Number: US 8,465,940 B2
- Document ID: US8465940B2
- Status: Granted
- Kind: B2
- Application number: 12/448,120
- Filed: 2007-12-13
- Published: 2010-03-11
- Granted: 2013-06-18
- Earliest claimed: 2006-12-14
- Assignees: Nippon Kayaku Kabushiki Kaisha
- Applicants: Yoshihiko Umegae; Reiko Machida; Hisako Takagi; Yayoi Irie; Takao Yokoyama; Toshio Tanabe
- Inventors: Yoshihiko Umegae; Reiko Machida; Hisako Takagi; Yayoi Irie; Takao Yokoyama; Toshio Tanabe
- Web page: https://patentgrep.com/patent/US8465940B2

## CPC

- G01N 33/66: involving blood sugars, e.g. galactose
- C12Q 1/005: {involving specific analytes or enzymes (including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence)}
- C12Q 1/26: involving oxidoreductase
- G01N 2333/902: Oxidoreductases (1.)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

By a method for measuring 1,5-anhydroglucitol, comprising the steps of: eliminating or converting glucose interfering with the measurement of 1,5-anhydroglucitol and/or a derivative thereof beforehand; and measuring 1,5-anhydroglucitol performed thereafter, wherein such glucose and/or a derivative thereof are/is eliminated or converted in whole blood as such without performing blood cell separation, an enzyme for measuring 1,5-anhydroglucitol is allowed to act on without performing blood cell separation, and 1,5-anhydroglucitol is electrochemically measured, it becomes possible to measure 1,5-anhydroglucitol using a small amount of whole blood without resort to a centrifuge or the like. Accordingly, this measurement method can be applied to rapid measurement of 1,5-anhydroglucitol at bedside or in a medical examination room or to home self-measurement thereof by a patient.

## Claims

### Claim 1

A method for measuring 1,5-anhydroglucitol in whole blood, comprising the steps of (1) enzymatically converting glucose or a derivative thereof, or glucose and a derivative thereof, that interferes with the measurement of 1,5-anhydroglucitol, to a substance that an enzyme for measuring 1,5-anhydroglycitol does not react with; and (2) electrochemically measuring 1,5-anhydroglucitol with the enzyme and an electrode, in said whole blood from which blood cells have not been separated.

### Claim 2 (depends on 1)

The method for measuring 1,5-anhydroglucitol according to claim 1, wherein the enzyme for measuring 1,5-anhydroglucitol is an oxidoreductase.

### Claim 3 (depends on 2)

The method for measuring 1,5-anhydroglucitol according to claim 2, wherein the oxidoreductase is pyranose oxidase, L-sorbose oxidase, or 1,5-anhydroglucitol dehydrogenase.

### Claim 4 (depends on 3)

The method for measuring 1,5-anhydroglucitol according to claim 3, wherein the oxidoreductase is derived from the genus Pseudomonas or Agrobacterium.

### Claim 5 (depends on 1, 2, 3, 4)

The method for measuring 1,5-anhydroglucitol according to any one of claims 1 to 4, wherein the 1,5-anhydroglucitol is electrochemically measured in the presence of a redox mediator.

### Claim 6 (depends on 5)

The method for measuring 1,5-anhydroglucitol according to claim 5, wherein the redox mediator is an osmium complex, a quinone compound, a ferrocene compound, a phenothiazine compound, a phenoxazine compound, a phenazine compound, an indophenol compound, a diphenylamine compound, or a phenol compound.

### Claim 7 (depends on 1)

The method for measuring 1,5-anhydroglucitol according to claim 1, wherein the 1,5-anhydroglucitol is electrochemically measured in the presence of a stabilizer.

### Claim 8 (depends on 7)

The method for measuring 1,5-anhydroglucitol according to claim 7, wherein the stabilizer is 2-sulfobenzoic acid or 3-sulfobenzoic acid.

### Claim 9 (depends on 1)

The method for measuring 1,5-anhydroglucitol according to claim 1, wherein the 1,5-anhydroglucitol is electrochemically measured by amperometry, coulometry, or cyclic voltammetry.

### Claim 10 (depends on 5)

The method for measuring 1,5-anhydroglucitol according to claim 5, wherein the 1,5-anhydroglucitol is electrochemically measured using an electrode having a working electrode for measuring 1,5-anhydroglucitol that contains an oxidoreductase for measuring 1,5-anhydroglucitol and a redox mediator, and a counter electrode.

### Claim 11 (depends on 10)

The method for measuring 1,5-anhydroglucitol according to claim 10, wherein the 1,5-anhydroglucitol is electrochemically measured using a differential electrode.

### Claim 12 (depends on 11)

The method for measuring 1,5-anhydroglucitol according to claim 11, wherein the differential electrode is an electrode having a working electrode for measuring 1,5-anhydroglucitol that contains an oxidoreductase for measuring 1,5-anhydroglucitol and a redox mediator, a working electrode for measuring a blank that contains a redox mediator but does not contain an oxidoreductase for measuring 1,5-anhydroglucitol, and a counter electrode.