# US 8,465,933 B2 · Method for screening human ABO blood group-binding lactobacilli

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 8,465,933 B2
- Document ID: US8465933B2
- Status: Granted
- Kind: B2
- Application number: 12/987,518
- Filed: 2011-01-10
- Published: 2011-05-05
- Granted: 2013-06-18
- Earliest claimed: 2004-12-01
- Assignees: Meiji Co., Ltd.
- Applicants: Tadao Saito; Yasushi Kawai; Hideaki Uchida; Katsunori Kimura; Kakuhei Isawa; Keisuke Furuichi
- Inventors: Tadao Saito; Yasushi Kawai; Hideaki Uchida; Katsunori Kimura; Kakuhei Isawa; Keisuke Furuichi
- Web page: https://patentgrep.com/patent/US8465933B2

## CPC

- A23C 9/1234: {characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.}
- C12Q 1/04: Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)}
- G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
- G01N 33/56911: {Bacteria}
- G01N 33/80: involving blood groups or blood types {or red blood cells (white blood cells G01N33/56972)}
- A23V 2400/145: Gasseri
- G01N 2333/335: from Lactobacillus (G)
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

Lactobacillus screening methods were carried out using surface plasmon resonance spectrums and human intestinal mucin and blood group antigens as probes. A trial to set selection criteria in the above-mentioned methods of screening for lactobacilli was made to adapt the methods to mass screening, and it was discovered that lactobacilli compatible with ABO blood groups can be screened by setting 100 RU as a criterion for judging bacterial binding under certain conditions. Using 238 lactobacillus strains, the above-mentioned screening methods and tests to judge their compatibility for the use of yogurt production were carried out, to at long last specifically discover bacillus strains compatible with blood groups A, B, and O.

## Claims

### Claim 1

A method of screening for a human blood group A-compatible lactobacillus, comprising:
(a) providing a candidate lactobacillus;
(b) determining ability of the candidate lactobacillus to recognize a human blood group A antigen, a human blood group B antigen, and a human blood group H antigen, wherein the recognizing ability is determined by measuring response units (RU) of surface plasmon resonance spectrum, wherein the human blood group A antigen comprises [GalNAcα1-3(Fucα1-2)Gal-]; and
(c) selecting the candidate lactobacillus as a human blood group A-compatible lactobacillus if:
(i) blood group A antigen-recognizing ability is 2,000 RU or higher;
(ii) blood group B antigen-recognizing ability is 100 RU or lower;
(iii) blood group H antigen-recognizing ability is 100 RU or lower;
(iv) ratio of the blood group A antigen-recognizing ability to the blood group B antigen-recognizing ability is 70 or higher;
(v) ratio of the blood group A antigen-recognizing ability to the blood group H antigen-recognizing ability is 100 or higher;
(vi) A-HCM-recognizing ability is 100 RU or higher; and
(vii) O-HCM-recognizing ability is 100 RU or lower.

### Claim 2 (depends on 1)

The method, according to claim 1, wherein the human blood group B antigen comprises [Galα1-3 (Fucα1-2)Gal-].

### Claim 3 (depends on 1)

The method, according to claim 1, wherein the human blood group H antigen comprises [Fucα1-2Gal-].

### Claim 4

A method of screening for a human blood group B-compatible lactobacillus, comprising:
(a) providing a candidate lactobacillus;
(b) determining ability of the candidate lactobacillus to recognize a human blood group A antigen, a human blood group B antigen, and a human blood group H antigen using surface plasmon resonance, wherein the recognizing ability is determined by measuring response units (RU) of surface plasmon resonance spectrum, wherein the human blood group B antigen comprises [Galα1-3(Fucα1-2)Gal-]; and
(c) selecting the candidate lactobacillus as a human blood group B-compatible lactobacillus if:
(i) blood group B antigen-recognizing ability is 2,000 RU or higher;
(ii) blood group A antigen-recognizing ability is 100 RU or lower;
(iii) blood group H antigen-recognizing ability is 100 RU or lower;
(iv) ratio of the blood group B antigen-recognizing ability to the blood group A antigen-recognizing ability is 50 or higher;
(v) ratio of the blood group B antigen-recognizing ability to the blood group H antigen-recognizing ability is 20 or higher; and
(vi) B-HCM-recognizing ability is higher than A-HCM-recognizing ability and O-HCM recognizing ability.

### Claim 5 (depends on 4)

The method, according to claim 4, wherein the human blood group A antigen comprises [GalNAcα1-3(Fucα1-2)Gal-].

### Claim 6 (depends on 4)

The method, according to claim 4, wherein the human blood group H antigen comprises [Fucα1-2Gal-].

### Claim 7

A method of screening for a human blood group O-compatible lactobacillus, comprising:
(a) providing a candidate lactobacillus;
(b) determining ability of the candidate lactobacillus to recognize a human blood group A antigen, a human group B antigen, and a human group H antigen using surface plasmon resonance, wherein the recognizing ability is determined by measuring response units (RU) of surface plasmon resonance spectrum, wherein the human blood group H antigen comprises [Fucα1-2Gal-]; and
(c) selecting the candidate lactobacillus as a human blood group O-compatible lactobacillus if:
(i) blood group H antigen-recognizing ability is 700 RU or higher;
(ii) blood group A antigen-recognizing ability is 100 RU or lower;
(iii) blood group B antigen-recognizing ability is 100 RU or lower;
(iv) ratio of the blood group H anti en-recognizing ability to the blood group A antigen-recognizing ability is 800 or higher;
(v) ratio of the blood group H antigen-recognizing ability to the blood group B antigen-recognizing ability is 20 or higher;
(vi) O-HCM-recognizing ability is 100 RU or higher; and
(vii) A-HCM-recognizing ability is 1,000 RU or lower.

### Claim 8 (depends on 7)

The method, according to claim 7, wherein the human blood group A antigen comprises [GalNAcα1-3(Fucα1-2)Gal-].

### Claim 9 (depends on 7)

The method, according to claim 7, wherein the human blood group B antigen comprises [Galα1-3(Fucα1-2)Gal-].