# US 8,465,932 B2 · Method for the determination of trichinella infections and diagnostic composition for such methods

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- Number: US 8,465,932 B2
- Document ID: US8465932B2
- Status: Granted
- Kind: B2
- Application number: 12/937,129
- Filed: 2009-06-16
- Published: 2011-02-10
- Granted: 2013-06-18
- Earliest claimed: 2008-06-27
- Assignees: Prionics AG
- Applicants: Alex Raber; Tina Haupt; Patrik Buholzer
- Inventors: Alex Raber; Tina Haupt; Patrik Buholzer
- Web page: https://patentgrep.com/patent/US8465932B2

## CPC

- G01N 33/5308: {for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites}
- G01N 2333/4353: from nematodes
- G01N 2800/26: Infectious diseases, e.g. generalised sepsis
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Method for the determination of Trichinella infections in an animal or a human, comprising the incubation with Trichinella antigens of a tissue sample taken from the animal or the human, the processing of the tissue sample, the addition of anti-antibodies to the tissue sample, and the verification of whether binding of the anti-antibodies to any antigen/antibody complexes present in the tissue sample has taken place, where the anti-antibodies added are anti-IgG antibodies and simultaneously anti-IgM antibodies.

## Claims

### Claim 1

A method for the determination of Trichinella infections in an animal or a human, comprising:
incubating a tissue sample taken from the animal or the human together with a Trichinella antigen that is capable of forming a Trichinella antibody/antigen complex with Trichinella antibodies that may be present in the tissue sample;
adding both anti-IgG antibodies and anti-IgM antibodies to the incubated tissue sample; and
testing whether a binding of both of the anti-IgG antibodies and the anti-IgM antibodies has taken place with a Trichinella antibody/antigen complex which may be present in the tissue sample.

### Claim 2 (depends on 1)

The method as claimed in claim 1, wherein the the Trichinella antigen that is capable of forming Trichinella antibody/antigen complex with Trichinella antibodies is Trichinella E/S antigen.

### Claim 3 (depends on 1)

The method as claimed in claim 1, wherein the Trichinella antigen employed is present in immobilized form.

### Claim 4 (depends on 1)

The method as claimed in claim 1, wherein labeled anti-IgG antibodies and anti-IgM antibodies are employed.

### Claim 5 (depends on 1)

The method as claimed in claim 1, wherein the anti-IgG antibodies are provided with a different label than anti-IgM antibodies.

### Claim 6 (depends on 1)

The method as claimed in claim 1, wherein the tissue sample is one or more selected from the group consisting of serum, plasma and meat juice.