# US 8,465,927 B2 · Detection of Neisseria meningitidis by loop mediated isothermal amplification

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- Number: US 8,465,927 B2
- Document ID: US8465927B2
- Status: Granted
- Kind: B2
- Application number: 12/743,115
- Filed: 2008-11-14
- Published: 2010-12-02
- Granted: 2013-06-18
- Earliest claimed: 2007-11-14
- Assignees: Belfast Health and Social Care Trust
- Applicants: Peter Coyle; Derek Fairley; James McKenna
- Inventors: Peter Coyle; Derek Fairley; James McKenna
- Web page: https://patentgrep.com/patent/US8465927B2

## CPC

- C12Q 1/689: for bacteria
- C12Q 1/6853: using modified primers or templates
- C12Q 2600/16: Primer sets for multiplex assays
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention provides a LAMP assay for detection of meningococcal disease, the test comprising at least one nucleic acid primer set capable of detecting Neisseria meningitides in a LAMP based molecular test, the primer set being chosen from the primer sets listed in Table 1 as LAMP SETS 1 to 12 comprising SEQUENCE IDs from ID:1 to ID:69. Each assay consists of a primer set including of one pair of forward (FIP) and reverse (BIP) inner primers, forward (F3) and reverse (B3) outer primers. The assay may also include loop forward (LF) and/or loop back (LB) primers to accelerate the reaction. Neisseria meningitides serotypes A, B, C, Y and W135 can be detected using the assay of the invention.

## Claims

### Claim 1

A method of detecting meningococcal disease comprising:
amplifying by isothermal amplification at least a portion of the ctrA gene of Neisseria meningitidis using one or both loop-mediated isothermal amplification (LAMP) primer sets selected from the group consisting of (a) and (b) as follows:
(a) the nucleotide sequences of LAMP SET 3 L3L3, SEQ ID NOS: 11 to 14, 16 and 17; and
(b) the nucleotide sequences of LAMP SET 3 L3L1, SEQ ID NOS: 11 to 16;
then detecting the amplified product.

### Claim 2 (depends on 1)

The method of claim 1, wherein the primer set is LAMP SET 3 L3L3 consisting of SEQ ID NOS: 11 to 14, 16 and 17.