# US 8,465,911 B2 · Native potato protein isolates

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- Number: US 8,465,911 B2
- Document ID: US8465911B2
- Status: Granted
- Kind: B2
- Application number: 12/513,971
- Filed: 2007-10-25
- Published: 2010-02-18
- Granted: 2013-06-18
- Earliest claimed: 2006-11-10
- Assignees: Coöperatie AVEBE U.A.
- Applicants: Marco Luigi Federico Giuseppin; Catrinus Van Der Sluis; Marc Christiaan Laus
- Inventors: Marco Luigi Federico Giuseppin; Catrinus Van Der Sluis; Marc Christiaan Laus
- Web page: https://patentgrep.com/patent/US8465911B2

## CPC

- A23L 29/206: of vegetable origin
- A23J 1/006: {from vegetable materials (A23J1/005, A23J1/12 and A23J1/14 take precedence)}
- A23J 1/16: from waste water of starch-manufacturing plant or like wastes
- A23J 3/14: Vegetable proteins
- A23L 2/52: Adding ingredients (adding preservatives A23B70/10)
- A23L 2/66: Proteins
- A23L 33/185: Vegetable proteins
- A61K 47/42: Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein (oligopeptides having up to five amino acids {A61K47/183}; polyamino acids A61K47/34)
- C07K 14/415: from plants
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The invention relates to a process for native potato protein isolation, to native potato protein isolates, to the use thereof, and to a food product comprising a native potato protein isolate. The invention provides a novel isolation process for obtaining highly pure native potato protein isolates having a glycoalkaloid concentration of less than 150 ppm.

## Claims

### Claim 1

Process for obtaining a native potato protein isolate comprising patatin and protease inhibitor, comprising
i) subjecting potato fruit juice to a flocculation by a divalent metal cation at a pH of 7-9;
ii) centrifuging the flocculated potato fruit juice, thereby forming a supernatant;
iii) subjecting the supernatant to expanded bed adsorption chromatography operated at a pH of less than 11 and a temperature of 5-35° C. using an adsorbent capable of binding potato protein, thereby adsorbing native potato protein to the adsorbent; and
iv) eluting at least one native potato protein isolate from the adsorbent with an eluent.

### Claim 2 (depends on 1)

Process according to claim 1, wherein said total native potato protein isolate has an isoelectric point above 4.8, a molecular weight of more than 4 kDa and a glycoalkaloid concentration of less than 150 ppm.

### Claim 3 (depends on 1)

Process according to claim 1, wherein said flocculation is carried out at a pH of 7.0-7.5.

### Claim 4 (depends on 1)

Process according to claim 1, wherein said expanded bed adsorption chromatography is operated at a pH of less than 10.

### Claim 5 (depends on 1)

Process according to claim 1, wherein the eluent has a pH of 4-12, preferably 5.5-11.0.

### Claim 6 (depends on 1)

Process according to claim 1, wherein the adsorbent is a mixed-mode adsorbent.

### Claim 7 (depends on 6)

Process according to claim 6, wherein a native potato protein patatin isolate is eluted at a pH of 5.7-8.7 and wherein a native potato protein protease inhibitor isolate is eluted at a pH of 5.8-12.0.

### Claim 8 (depends on 6)

Process according to claim 6, wherein a native potato protein patatin isolate is eluted at a pH of 5.8-6.2 and wherein a native potato protein protease inhibitor isolate is eluted at a pH of 6.0-9.5.

### Claim 9 (depends on 7)

Process according to claim 7, wherein the native potato protein protease inhibitor isolate is further processed into at least one isolate selected from the group consisting of a protease inhibitor I isolate, a carboxypeptidase inhibitor isolate, a protease inhibitor IIa and IIb isolate, and a protease inhibitor A5 isolate by ion exchange or gel permeation chromatography.

### Claim 10 (depends on 6)

Process according to claim 6, wherein the mixed-mode adsorbent is used in selective elution mode.

### Claim 11 (depends on 6)

Process according to claim 6, wherein the mixed-mode adsorbent is used in selective adsorption mode.

### Claim 12 (depends on 1)

Process according to claim 1, which process is followed by an ultrafiltration, at a pH of less than 6.2 to remove glycoalkaloids.

### Claim 13 (depends on 12)

Process according to claim 12, at a pH value to increase the flux.

### Claim 14 (depends on 1)

Process according to claim 1, which process is followed by a spray drying or freeze concentrating step.