# US 8,465,787 B2 · Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids

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- Number: US 8,465,787 B2
- Document ID: US8465787B2
- Status: Granted
- Kind: B2
- Application number: 13/428,133
- Filed: 2012-03-23
- Published: 2012-07-12
- Granted: 2013-06-18
- Earliest claimed: 2008-06-20
- Assignees: E. I. Du Pont de Nemours and Company
- Applicants: Brian McGonigle; Anthony J. Kinney; Howard Glenn Damude
- Inventors: Brian McGonigle; Anthony J. Kinney; Howard Glenn Damude
- Web page: https://patentgrep.com/patent/US8465787B2

## CPC

- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- A23D 9/00: Other edible oils or fats, e.g. shortenings or cooking oils (preservation of other edible oils A23B20/30)
- C12N 9/1029: {transferring groups other than amino-acyl groups (2.3.1)}
- C12P 7/6427: Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23D: EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS (preservation thereof A23B20/00; production, refinement or preservation of animal or vegetable fats or oils C11B, C11C)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding novel delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.

## Claims

### Claim 1

Soybean oil comprising cis-9-eicosenoic acid, cis-11,14-eicosadienoic acid, cis-11,14,17-eicosatrienoic acid, cis-9-octadecanoic acid, cis-9,12-octadecanoic acid, and cis-9,12,15-octadecatrienoic acid, wherein the percent delta-9 conversion of cis-9-octadecanoic acid, cis-9,12-octadecadienoic acid, and cis-9,12,15-octadecatrienoic acid to cis-9-eicosenoic acid, cis-11,14-eicosadienoic acid, and cis-11,14,17-eicosatrienoic acid is 29.18%-44.55%.