# US 8,465,781 B2 · Preparation and use of a plant extract from Solanum glaucophyllum with an enriched content of 1,25-dihydroxyvitamin D3 glycosides and quercetin glycosides

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- Number: US 8,465,781 B2
- Document ID: US8465781B2
- Status: Granted
- Kind: B2
- Application number: 12/988,598
- Filed: 2008-04-21
- Published: 2011-02-17
- Granted: 2013-06-18
- Earliest claimed: 2008-04-21
- Assignees: Herbonis AG
- Applicants: Sabrina Autzen; Heinrich Bachmann
- Inventors: Sabrina Autzen; Heinrich Bachmann
- Web page: https://patentgrep.com/patent/US8465781B2

## CPC

- A61K 36/81: Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 15/00: Drugs for genital or sexual disorders (for disorders of sex hormones A61P5/24); Contraceptives
- A61P 15/14: for lactation disorders, e.g. galactorrhoea
- A61P 19/00: Drugs for skeletal disorders
- A61P 19/08: for bone diseases, e.g. rachitism, Paget's disease
- A61P 19/10: for osteoporosis
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to the preparation of an enriched and equilibrated plant extract from Solanum glaucophyllum with an enriched content of 1,25-dihydroxyvitamin D3 glycosides and flavonols, particularly quercetin glycosides. The present invention particularly describes a method for preparation of such a plant extract either in industrial or in laboratory scale. The present invention furthermore describes the use of such a plant extract or similar (synthetic) compositions for the prevention and treatment of bone mass reduction-related diseases, such as Osteopenia or Osteoporosis, for the prevention and treatment of Tibial Dyschondroplasia, preferably in poultry, for the treatment of milk fever, and as a dietary supplement for human or veterinary use.

## Claims

### Claim 1

A method for preparation and purification of an enriched plant extract from Solanum glaucophyllum having an enriched content of 1,25-dihydroxyvitamin D3 glycosides and quercetin glycosides, wherein the method comprises the following steps:
(a) extracting plants or parts thereof from the species Solanum Glaucophyllum using an alcohol/water mixture having an alcohol/water ratio (%) of 75/25 to 25/75 (v/v), the alcohol selected from the group consisting of methanol, ethanol and isopropanol; and
(b) purifying the plant extract obtained according to step a), using the steps:
b1) applying the plant extract obtained according to step a) to a column comprising a non-ionic polymer resin selected from the group consisting of polystyrene, styrene-divinyl-benzene copolymers, acrylic ester polymers and polyphenolic resins;
b2) washing the column with water;
b3) eluting the enriched plant extract from the column; and
b4) concentrating and/or drying the enriched plant extract.

### Claim 2 (depends on 1)

The method according to claim 1, wherein step a) comprises the step of grinding, percolating and/or macerating the plants or parts from the species Solanum glaucophyllum.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the alcohol/water mixture is an ethanol/water mixture having a ratio (%) of about 25/75 or of about 75/25 ethanol/water (v/v) for percolation type extractions, or alternatively of about 65/35 ethanol/water (v/v) for maceration type extractions.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the ratio of the alcohol/water mixture to the plants or parts thereof (%) is about 4-40 (v/w), for percolation type extractions, or alternatively about 3-7 (v/w) for maceration type extractions.

### Claim 5 (depends on 1)

The method according to claim 1, wherein step a) is carried out in a temperature range of about 20° C. to about 85° C. and wherein the solvent in step a) is adjusted to a specific pH-value of about 4.0 to about 8.0.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the plant extract obtained according to step a) and/or b) is concentrated to a content of about 85-75% water and about 15-25% non-volatile matter.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the extract obtained according to step a) is subjected to a treatment at a temperature from about 100° C. to about 145° C.

### Claim 8 (depends on 1)

The method according to claim 1, wherein the non-ionic polymer resin comprises styrene-divinyl-benzene copolymers.

### Claim 9 (depends on 1)

The method according to claim 1, wherein the extract obtained according to step a) and/or b) is spray-dried, band dried or lyophilized.