# US 8,465,754 B2 · Bordetella parapertussis whole-cell vaccine composition

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- Number: US 8,465,754 B2
- Document ID: US8465754B2
- Status: Granted
- Kind: B2
- Application number: 12/601,795
- Filed: 2008-05-28
- Published: 2010-10-14
- Granted: 2013-06-18
- Earliest claimed: 2007-05-28
- Assignees: Kitasato Daiichi Sankyo Vaccine Co., Ltd.
- Applicants: Mineo Watanabe; Eiji Komatsu
- Inventors: Mineo Watanabe; Eiji Komatsu
- Web page: https://patentgrep.com/patent/US8465754B2

## CPC

- A61K 39/099: {Bordetella}
- A61P 31/04: Antibacterial agents
- A61K 2039/521: {inactivated (killed)}
- A61K 2039/545: {characterised by the dose, timing or administration schedule}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

An objective of the present invention is to provide a whole-cell bacterial vaccine composition for preventing whooping cough caused by Bordetella parapertussis, comprising whole cells, whole-cell homogenate, or cell lysate of B. parapertussis as an immunogen, and methods for producing them.

## Claims

### Claim 1

A method for preventing whooping cough caused by B. parapertussis in a subject, the method comprising steps of:
(a) culturing B. parapertussis cells at 35° C. to 37° C. using filamentous hemagglutinin (FHA) as an index to produce a B. parapertussis culture preparation with a final cell concentration of 1010 cells/mL;
(b) inactivating the B. parapertussis cells by adding a formalin solution comprising formaldehyde at a final concentration of 0.2% to the B. parapertussis culture preparation; and
(c) administering the inactivated B. parapertussis cells as whole cells, as a whole-cell homogenate thereof, or as a cell lysate thereof to the subject.

### Claim 2

A method for inducing immunity against B. parapertussis in a subject, the method comprising steps of:
(a) culturing B. parapertussis cells at 35° C. to 37° C. using filamentous hemagglutinin (FHA) as an index to produce a B. parapertussis culture preparation with a final cell concentration of 1010 cells/mL;
(b) inactivating the B. parapertussis cells by adding a formalin solution comprising formaldehyde at a final concentration of 0.2% to the B. parapertussis culture preparation; and
(c) administering the inactivated B. parapertussis cells as whole cells, as a whole-cell homogenate thereof, or as a cell lysate thereof to the subject.