Plants having enhanced yield-related traits and a method for making the same
AssigneeCropDesign N.V.
InventorValerie Frankard
Life of this patent
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Abstract
The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for increasing seed yield in plants by increasing expression in a plant of a nucleic acid sequence encoding a Dwarf1 (DWF1) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding a DWF1 polypeptide, which plants have increased seed yield relative to control plants. The invention also provides constructs useful in performing the methods of the invention.
Claims28
A method for increasing seed yield in plants relative to control plants, comprising increasing expression in a plant of a nucleic acid sequence encoding a Dwarf1 (DWF1) polypeptide, wherein the DWF1 polypeptide comprises from N-terminus to C-terminus:
- (i) a transmembrane domain;
- (ii) an FAD-binding domain;
- and (iii) a substrate-binding domain having at least 98% sequence identity to the substrate-binding domain of SEQ ID NO:
- 29, and selecting for a plant having increased seed yield relative to a corresponding control plant.
A method for the production of a transgenic plant having increased seed yield relative to control plants, comprising:
- (a) introducing and expressing in a plant a nucleic acid sequence encoding a DWF1 polypeptide, wherein the DWF1 polypeptide comprises from N-terminus to C-terminus:
- (i) a transmembrane domain;
- (ii) an FAD-binding domain;
- and (iii) a substrate-binding domain having at least 98% sequence identity to the substrate-binding domain of SEQ ID NO:
- 29;
- and
- (b) cultivating the plant or plant cell under conditions promoting plant growth and development, and
- (c) selecting for a plant having increased seed yield relative to a corresponding control plant.
A transgenic plant having increased seed yield relative to control plants, resulting from increased expression of a nucleic acid sequence encoding a DWF1 polypeptide, wherein the DWF1 polypeptide comprises from N-terminus to C-terminus:
- (i) a transmembrane domain;
- (ii) an FAD-binding domain;
- and (iii) a substrate-binding domain having at least 98% sequence identity to the substrate-binding domain of SEQ ID NO:
- 29, or a transgenic plant cell derived from said transgenic plant.
An isolated polypeptide having at least 99% sequence identity to the amino acid sequence of SEQ ID NO:
- 2 or 34.
An isolated polypeptide comprising from N-terminus to C-terminus:
- (i) a transmembrane domain;
- (ii) a FAD-binding domain;
- and
- (iii) a substrate-binding domain having at least 99% or more sequence identity to the substrate-binding domain of SEQ ID NO:
- 29.
An isolated nucleic acid comprising the nucleic acid sequence of SEQ ID NO:
- 1 or 33 or encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 2 or 34.
A construct comprising:
- a) a nucleic acid sequence encoding a DWF1 polypeptide, wherein the nucleic acid has at least 90% sequence identity to the nucleic acid sequence of SEQ ID NO:
- 1 or 33;
- b) one or more control sequences capable of driving expression of the nucleic acid sequence of (a);
- and optionally
- c) a transcription termination sequence.
A construct comprising:
- a) a nucleic acid sequence encoding a DWF1 polypeptide, wherein the DWF1 polypeptide comprises from N-terminus to C-terminus:
- (i) a transmembrane domain;
- (ii) an FAD-binding domain;
- and (iii) a substrate-binding domain having at least 99% sequence identity to the substrate-binding domain of SEQ ID NO:
- 29;
- b) one or more control sequences capable of driving expression of the nucleic acid sequence of (a);
- and optionally
- c) a transcription termination sequence.
Dependencies are inferred from the claim text.