B7-H1 antibodies and method of use
AssigneeMayo Foundation for Medical Education and Research
InventorLieping Chen
Life of this patent
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Abstract
The invention provides novel polypeptides useful for co-stimulating T cells, isolated nucleic acid molecules encoding them, vectors containing the nucleic acid molecules, and cells containing the vectors. Also included are methods of making and using these co-stimulatory polypeptides.
Claims6
A method for inhibiting a co-stimulatory effect of B7-H1 on T-cells, comprising:
- contacting said B7-H1 with an isolated antibody that binds specifically to a B7-H1 polypeptide which comprises
- (a) amino acids having the sequence set forth in SEQ ID NO:1;
- or
- (b) amino acids that are encoded by a polynucleotide comprising nucleotides having the sequence set forth in SEQ ID NO:2,
- wherein the contacting comprises culturing the antibody with the T-cell in vitro, to thereby inhibit a co-stimulatory effect of B7-H1 on T-cells.
An isolated antibody that binds specifically to a B7-H1 polypeptide which comprises
- (a) amino acids having the sequence set forth in SEQ ID NO:3;
- or
- (b) amino acids that are encoded by a polynucleotide comprising nucleotides having the sequence set forth in SEQ ID NO:4.
An antibody fragment that binds specifically to a B7-H1 polypeptide which comprises
- (a) amino acids having the sequence set forth in SEQ ID NO:3;
- or
- (b) amino acids that are encoded by a polynucleotide comprising nucleotides having the sequence set forth in SEQ ID NO:4.
A method for inhibiting a co-stimulatory effect of B7-H1 on T-cells, comprising:
- contacting said B7-H1 with an isolated antibody or an antigen binding fragment thereof which binds specifically to a soluble B7-H1 polypeptide which is not membrane-bound, wherein said soluble B7-H1 polypeptide comprises amino acid domain(s) having the sequence(s) shown in FIG. 2A (SEQ ID NO:1) such that the polypeptide is soluble, to thereby inhibit a co-stimulatory effect of B7-H1 on T-cells.
Dependencies are inferred from the claim text.