Methods and arrays for target analyte detection and determination of target analyte concentration in solution

A method of detecting a target analyte in a sample, the method comprising:

• a) providing a sample and a solid support, said sample comprising a biomolecule target analyte, said solid support comprising at least 1000 assay sites, each assay site having a defined volume between 10 attoliters and 50 picoliters;
• b) contacting said solid support with said sample so that assay sites of the solid support contain biomolecule target analyte that is bound to capture components within the assay sites, and such that the ratio of a total number of biomolecule target analyte molecules contained within the assay sites to the total number of assay sites contacted with said sample is less than 1:5;
• and
• c) determining the number of assay sites contacted with said sample which contain a biomolecule target analyte.