Polyvalent chimeric rubella virus-based vaccines
AssigneeNational Health Research Institutes
InventorsYen-Hung Chow, Charles Dwo-Yuan Sia, Pele Choi-Sing Chong, Kuang-Nan Hsiao
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Abstract
A chimeric viral particle that comprises a RV fusion gene is disclosed. The RV fusion gene comprises a first nucleotide sequence encoding a RV that is devoid of RV E1 protein, and a second nucleotide sequence that linked in translation frame to the first nucleotide sequence and encodes a humoral immunogenic viral protein. The chimeric viral particle is free of RV E1 protein-encoding gene. A virus packaging cell that generates the chimeric viral particle comprising a RV fusion gene and an isolated expression vector comprising a RV fusion gene linked in translation frame to a promoter are also disclosed.
Claims20
A chimeric viral particle whose genome comprises:
- a) a first nucleotide sequence encoding rubella virus (RV) proteins, wherein the proteins encoded by the first nucleotide sequence are devoid of RV E1 protein;
- and
- b) a second nucleotide sequence, linked in translation frame to the first nucleotide sequence, encoding respiratory syncytial virus (RSV) F protein;
- wherein the chimeric viral particle is free of RV E1 protein-encoding gene.
A chimeric viral particle whose genome comprises:
- a) a first nucleotide sequence encoding RV proteins, wherein the proteins encoded by the first nucleotide sequence are devoid of RV E1 protein;
- and
- b) a second nucleotide sequence, linked in translation frame to the first nucleotide sequence, encoding an immunogenic foreign viral protein;
- wherein the chimeric viral particle is free of RV E1 protein-encoding gene.
An isolated expression vector comprising:
- i) a promoter;
- ii) a first nucleotide sequence, linked in translation frame to the promoter, encoding RV proteins, wherein the proteins encoded by the first nucleotide sequence are devoid of E1 protein;
- and
- iii) a second nucleotide sequence, linked in translation frame to the first nucleotide sequence, encoding an immunogenic foreign viral protein;
- wherein the expression vector is devoid of RV E1 protein-encoding nucleotide sequence.
Dependencies are inferred from the claim text.