Collagen producing plants and methods of generating and using same
AssigneeCollPlant Ltd.
InventorsOded Shoseyov, Hanan Stein
Life of this patent
Estimating the term…
Abstract
A method of producing collagen in a plant and plants producing collagen are provided. The method is effected by expressing in the plant at least one type of a collagen alpha chain in a manner enabling accumulation of the collagen alpha chain in a subcellular compartment devoid of endogenous P4H activity, thereby producing the collagen in the plant.
Claims15
A method of producing collagen in a plant or an isolated plant cell comprising targeting to a vacuole of the plant or the isolated plant cell the collagen alpha 1 chain as set forth in SEQ ID NO:
- 3 and an exogenous prolyl-4-hydroxylase (P4H) so as to allow hydroxylation of the collagen alpha 1 chain by said exogenous P4H, thereby producing the collagen in the plant.
A method of producing collagen, said method comprising:
- (a) providing a plant system comprising:
- a first genetically modified plant comprising in a vacuole thereof.
- (i) the collagen alpha 1 chain as set forth in SEQ ID NO:
- 3;
- and
- (ii) an exogenous P4H;
- and
- a second genetically modified plant comprising in a vacuole thereof:
- (i) the collagen alpha 2 chain as set forth in SEQ ID NO:
- 6;
- and
- (ii) an exogenous P4H;
- (b) crossing said first plant and said second plant;
- and
- (c) selecting progeny expressing said collagen alpha 1 chain and said collagen alpha 2 chain thereby producing collagen.
A method of producing collagen comprising:
- (a) providing the plant system comprising:
- a first genetically modified plant comprising in a vacuole thereof:
- (i) the collagen alpha 1 chain as set forth in SEQ ID NO:
- 3;
- and
- (ii) the collagen alpha 2 chain as set forth in SEQ ID NO:
- 6;
- and
- a second genetically modified plant comprising in a vacuole thereof an exogenous P4H;
- and
- (b) crossing said first plant and said second plant and selecting progeny expressing the collagen alpha 1 chain, the collagen alpha 2 chain and said P4H thereby producing collagen.
Dependencies are inferred from the claim text.