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US 8,450,471 B2Granted
TAL effector-mediated DNA modification
AssigneesRegents of the University of Minnesota, Iowa State University Research Foundation, Inc.
InventorsDaniel F. Voytas, Adam J. Bogdanove, Feng Zhang
Life of this patent
Estimating the term…
Abstract
Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided.
Claims5
An article of manufacture comprising:
- (a) a nucleic acid encoding a first transcription activator-like (TAL) effector endonuclease monomer comprising a plurality of TAL effector repeat sequences and a FokI endonuclease domain, wherein each of said plurality of TAL effector repeat sequences comprises a repeat-variable diresidue, and wherein said first TAL effector endonuclease monomer is capable of binding to a first half-site sequence of a target DNA, and
- (b) a nucleic acid encoding a second TAL effector endonuclease monomer comprising a plurality of TAL effector repeat sequences and a FokI endonuclease domain, wherein each of said plurality of TAL effector repeat sequences of said second TAL effector endonuclease monomer comprises a repeat-variable diresidue, and wherein said first TAL effector endonuclease monomer is capable of binding to a second half-site sequence of said target DNA,
- wherein said first half-site sequence and said second half-site sequence are different and are separated by a spacer sequence, and wherein said first TAL effector endonuclease monomer and said second TAL effector endonuclease monomer are capable of forming a dimer that cleaves said target DNA within a living cell when said first TAL effector endonuclease monomer is bound to said first half-site sequence and said second TAL effector endonuclease monomer is bound to said second half-site sequence.
Dependencies are inferred from the claim text.