Method for the determination of the formation of endothelins for medical diagnostic purposes, and antibodies and kits for carrying out such a method
AssigneeB.R.A.H.M.S GmbH
InventorsAndreas Bergmann, Joachim Struck
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Abstract
Described herein is an in vitro method for the determination of the formation of endothelins in serious diseases, in particular cardiovascular diseases, inflammations, sepsis and cancer, in whole blood, plasma or serum of a human patient, in which relatively long-lived peptide fragments of the processed primary prepro- or proendothelins are determined which contain neither the actual biologically active endothelin nor its direct precursor big endothelin. In particular, disclosed are antibodies and kits for selectively detecting these fragments.
Claims14
An isolated antibody which binds specifically to a peptide consisting of amino acids (i) 184-203 or (ii) 200-212 of preproendothelin-1 (SEQ ID NO:
- 1).
A kit for, detecting C-terminal fragments of amino acids 168-212 of preproendothelin-1 (SEQ ID NO:
- 1), said kit comprising
- (a) a first antibody selected from antibodies that specifically bind to (i) a peptide consisting of amino acids 168-181 or (ii) antibodies that specifically bind to a peptide consisting of amino acids 184-203 of preproendothelin-1 (SEQ ID NO:
- 1);
- and
- (b) a second antibody selected from antibodies that specifically bind to a peptide consisting of amino acids 184-203 or antibodies that specifically bind to a peptide consisting of amino acids 200-212 of preproendothelin-1 (SEQ ID NO:
- 1), wherein said second antibody is different from said first antibody.
Dependencies are inferred from the claim text.