Targeting pseudotyped retroviral vectors
AssigneeThe Regents of the University of California
InventorsIrvin S.Y. Chen, Kouki Morizono
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Abstract
The present invention provides targeted lentiviral vectors that are psuedotyped with mutated Sindbis envelopes. For example, mutations in the Sindbis E2 protein are used to alter viral titer, specificity, specificity index, tropism, and susceptibility to host immune response. Typically, one or more of the E1, E2, or E3 proteins can be mutated at one or more amino acid positions. The psuedotyped, targeted lentiviral vectors of the invention are used to transduce heterologous genes into a cell and can be used for in vivo and ex vivo therapeutic applications, as well as for diagnostic and research tool applications.
Claims8
An isolated pseudotyped retrovirus comprising mutant Sindbis virus E2 and E3 proteins, wherein the mutant Sindbis virus E2 protein comprises an IgG Fc binding ZZ domain polypeptide and the sequence SLKQ of a SINDBIS virus E2 protein is replaced with the sequence AAAA and wherein the mutant Sindbis virus E3 protein comprises a deletion of the sequence RSKR of a Sindbis virus E3 protein.
Dependencies are inferred from the claim text.