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AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsAkinori Matsushika, Shigeki Sawayama, Hiroyuki Inoue, Keisuke Makino, Tsutomu Kodaki, Seiya Watanabe
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Genetic recombinant yeast expressing xylose reductase (XR), (wild-type or mutant) xylitol dehydrogenase (XDH), and xylulokinase (XK) and a method for highly efficiently producing ethanol from xylose using the yeast are provided. Pichia stipitis-derived XR and (wild-type or modified-type) XDH genes and Saccharomyces cerevisiae-derived XK gene were introduced via chromosomal integration. Thus, a genetic recombinant yeast having a high xylose fermentation rate, being capable of producing ethanol from xylose in high yields, and having high xylose fermentability in the presence of glucose, as well as a method using the recombinant yeast for highly efficiently producing ethanol from xylose or a saccharified solution from lignocellulose-based biomass are provided. Furthermore, a method for improving the xylose fermentability of the genetic recombinant yeast of the present invention via acclimatization treatment is also provided herein.
A genetic recombinant yeast capable of producing ethanol from xylose, in which a xylose reductase gene, a xylitol dehydrogenase gene, and a xylulokinase gene are introduced by chromosomal integration, wherein the xylose reductase gene, the xylitol dehydrogenase gene, and the xylulokinase gene are each expressed by a PGK promoter which constantly expresses each gene and the genetic recombinant yeast is prepared from strain IR-2 (FERM BP-754).
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