Methods and compositions for the production of monoclonal antibodies

A method for the in vitro production of monoclonal antibodies comprising:

• a) generating activated CD4+ T cells by incubating T cells with a composition comprising a first, second, and third colloidal metal particle joined together by a scaffolding molecule, wherein a major histocompatibility protein-II (MHC-II) is bound to the first particle, a co-stimulatory B7 protein is bound to the second particle, and a structural protein is bound to the third particle, and wherein an antigen is bound to the MHC-II;
• b) generating immunized B cells by incubating B cells with the same antigen as the antigen bound to the MHC-II;
• c) generating IgG positive B cells by incubating the activated CD4+ T cells with the immunized B cells;
• and
• d) generating a monoclonal antibody producing cell by immortalizing the IgG positive B cells.