Protein synthesis modulators
AssigneeRib-X Pharmaceuticals, Inc.
InventorBrian T. Wimberly
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Abstract
The invention provides a high resolution three-dimensional structure of a deacylated transfer RNA protein synthesis modulator in association with a large ribosomal subunit. The protein synthesis modulator binds at least a portion of the E-site of a large ribosomal subunit. The invention provides methods for designing and/or identifying analogs of candidate molecules, for example, analogs or derivatives of the protein synthesis modulator, that bind and/or modulate the protein biosynthetic activity of the ribosome.
Claims15
A method of identifying a molecule that binds to a large ribosomal subunit, the method comprising the steps of:
- (a) providing a molecular model of at least a portion of an RNA microhelix binding site of a large subunit of a ribosome created from (i) the atomic co-ordinates for the Haloarcula marismortui large ribosomal subunit found on Replacement Disk 1 under file name microhelix.txt or microhelixa.txt, or (ii) atomic co-ordinates derived by molecular modeling from the Haloarcula marismortui atomic co-ordinates found on Replacement Disk 1 under file name microhelix.txt or microhelixa.txt;
- (b) using the molecular model to identify a candidate molecule that binds to at least a portion of the RNA microhelix binding site of the molecular model, wherein the RNA microhelix binding site comprises at least a portion of the E-site of the large ribosomal subunit;
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- (c) producing the candidate molecule identified in step (b).
Dependencies are inferred from the claim text.