Method and system for imaging high density biochemical arrays with sub-pixel alignment
AssigneeComplete Genomics, Inc.
InventorsBryan P. Staker, Craig E. Uhrich
Life of this patent
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Abstract
A method and associated system for imaging high density biochemical arrays comprises one or more imaging channels that share a common objective lens and a corresponding one or more time delay integration-type imaging cameras with optical alignment mechanisms that permit independent inter-channel and intra-channel adjustment of each of four degrees: X, Y, rotation and scale. The imaging channels are configured to independently examine different spectra of the image of the biochemical arrays.
Claims26
A method for aligning an imaging system comprising a microscope objective and a camera, the method comprising:
- (a) collecting light emitted from a sample on an x-y positioning stage;
- (b) obtaining an image of the sample along a rotational axis of symmetry of the microscope objective;
- (c) calculating a first angle between the camera and the sample around the rotational axis of symmetry;
- (d) calculating a second angle between the sample and the x-y positioning stage around the axis of rotational symmetry;
- (e) rotating the sample by the second angle to align the sample with the x-y positioning stage;
- and
- (f) rotating the camera by the sum of the first angle and the second angle to align the camera with the x-y positioning stage.
An imaging system comprising:
- a microscope objective;
- a camera configured to produce an image from scanning of a sample along a scanning axis normal to an axis of rotational symmetry of the microscope objective;
- and
- a lateral offset system along the axis of rotational symmetry, the lateral offset system configured to independently shift the position of the image in the camera in a plane normal to the scanning axis, wherein the lateral offset system comprises an offset plate mounted to a galvo mechanism.
An imaging system comprising:
- a microscope objective;
- a camera configured to produce an image from scanning of a sample along a scanning axis normal to an axis of rotational symmetry of the microscope objective;
- and
- a lateral offset system along the axis of rotational symmetry, the lateral offset system configured to independently shift the position of the image in the camera in a plane normal to the scanning axis, wherein the lateral offset system comprises a rotational stage and an x-y positioning stage, and wherein the camera is mounted on the rotational stage and the x-y positioning stage.
An imaging system comprising:
- a microscope objective;
- a camera configured to produce an image from scanning of a sample along a scanning axis normal to an axis of rotational symmetry of the microscope objective;
- and
- a lateral offset system along the axis of rotational symmetry, the lateral offset system configured to independently shift the position of the image in the camera in a plane normal to the scanning axis further comprising a rotational stage that supports the camera, the rotational stage being rotatable around the axis of rotational symmetry.
An imaging system comprising:
- a microscope objective;
- a camera configured to produce an image from scanning of a sample along a scanning axis normal to an axis of rotational symmetry of the microscope objective;
- and
- a lateral offset system along the axis of rotational symmetry, the lateral offset system configured to independently shift the position of the image in the camera in a plane normal to the scanning axis, wherein the camera is a first camera and the lateral offset system is a first lateral offset system, and wherein the imaging system further comprises:
- a first imaging channel comprising the first camera and the first lateral offset system;
- a second imaging channel comprising a second camera and a second lateral offset system;
- a beam splitter configured to direct a first selected spectrum of the image to the first imaging channel and to direct a second selected spectrum of the image to the second imaging channel.
Dependencies are inferred from the claim text.