Back to results
US 8,426,200 B2Granted
Neuronal differentiation of stem cells
AssigneeRegents of the University of Minnesota
InventorsCatherine Verfaillie, Yuehua Jiang
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions and methods for culturing stem cells, such that neuronal differentiation can be achieved.
Claims4
A method for inducing stem cells to differentiate into neuronal cells comprising:
- a) culturing said stem cells with basic fibroblast growth factor;
- b) culturing the cells of step a) with fibroblast growth factor 8 and Sonic Hedgehog;
- c) culturing the cells of step b) with brain-derived neurotrophic factor;
- and
- d) co-culturing the cells of step c) with astrocytes;
- wherein said cells are cultured according to steps 1) through d) for at least seven days at each step.
3 more elements
A method for inducing cells to differentiate into neuronal cells comprising co-culturing the cells with astrocytes, said cells having gone through the steps of
- a) culturing stem cells with basic fibroblast growth factor;
- b) culturing the cells of step a) with fibroblast growth factor 8 and Sonic Hedgehog;
- and
- c) culturing the cells of step b) with brain-derived neurotrophic factor;
- wherein said cells are cultured according to steps a) through c) for at least seven days at each step.
2 more elements
Dependencies are inferred from the claim text.