Attenuated recombinant alphaviruses incapable of replicating in mosquitoes and uses thereof
AssigneeBoard of Regents, The University of Texas System
InventorsScott C. Weaver, Ilya V. Frolov, Elena Frolova
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Abstract
The present invention discloses an attenuated recombinant alphavirus that is incapable of replicating in mosquito cells and of transmission by mosquito vectors. These attenuated alphavirus may include but is not limited to Western Equine Encephalitis virus, Eastern equine encephalitis virus, Venezuelan equine encephalitis virus or Chikungunya virus. The present invention also discloses the method of generating such alphaviruses and their use as immunogenic compositions.
Claims15
An attenuated recombinant alphavirus comprising:
- an alphavirus nucleic acid, having (i) an insertion of an internal ribosomal entry site of encephalomyelocarditis virus (EMCV IRES) between the end of nonstructural protein 4 (nsP4) coding sequence and initiating AUG of a subgenomic RNA coding sequence of the alphavirus, and (ii) an inactivated subgenomic promoter, wherein the alphavirus is attenuated.
An expression vector comprising an alphavirus nucleic acid having (i) an insertion of an internal ribosomal entry site of encephalomyelocarditis virus (EMCV IRES) between the end of nonstructural protein 4 (nsP4) coding sequence and initiating AUG of a subgenomic RNA coding sequence of the alphavirus, and (ii) an inactivated subgenomic promoter.
Dependencies are inferred from the claim text.