Fungal transcriptional activator useful in methods for producing polypeptides
AssigneeNovozymes A/S
InventorsCarsten M. Hjort, Cees A. M. J. J. Van den Hondel, Peter J. Punt, Frank H. J. Schuren, Tove Christensen
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Abstract
The present disclosure relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The disclosure also relates to nucleic acid constructs, vectors and host cells including the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.
Claims17
A host cell for the production of a desired polypeptide, wherein the host cell comprises:
- (a) a first nucleic acid sequence that encodes a polypeptide having an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO:49, wherein the nucleic acid sequence is altered such that the host cell produces less protease compared to a similar cell with a nucleic acid sequence that encodes a polypeptide having an amino acid sequence of SEQ ID NO:49 when cultured under the same conditions, and
- (b) an inducible promoter operably linked to the nucleic acid sequence, and a promoter sequence that binds a transcriptional activator encoded by the nucleic acid sequence, operably linked to a nucleic acid sequence encoding the desired polypeptide, wherein the inducible promoter and promoter sequence can be induced simultaneously or sequentially.
A host cell for the production of a desired polypeptide, wherein the host cell comprises:
- (a) a first nucleic acid sequence encoding a polypeptide having an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO:49, wherein the nucleic acid sequence is altered or inactivated such that the cell produces a reduced amount of protease, and
- (b) an inducible promoter operably linked to the nucleic acid sequence, and a promoter sequence that binds a transcriptional activator encoded by the nucleic acid sequence, operably linked to a nucleic acid sequence encoding the desired polypeptide, wherein the inducible promoter and promoter sequence can be induced simultaneously or sequentially.
Dependencies are inferred from the claim text.