Method and device for identification of nucleated red blood cells from a maternal blood sample
AssigneeCellscape Corporation
InventorsBhairavi Parikh, Michael D. Brody, James Stone, Jonathan D. Halderman
Life of this patent
Estimating the term…
Abstract
A method for concentrating and isolating nucleated cells, such as a maternal and fetal nucleated red blood cells (NRBC's), in a maternal whole blood sample. The invention also provides methods and apparatus for preparing to analyze and analyzing the sample for identification of fetal genetic material as part of prenatal genetic testing. The invention also pertains to methods and apparatus for discriminating fetal nucleated red blood cells from maternal nucleated red blood cells obtained from a blood sample taken from a pregnant woman.
Claims8
A method of enriching for fetal nucleated cells from a maternal blood sample, the method comprising:
- passing a maternal blood sample containing maternal nucleated cells and fetal nucleated cells through a leukocyte depletion filter;
- retaining nucleated cells including leukocytes on the filter;
- and
- eluting nucleated cells from the filter with an elution buffer wherein the nucleated cells include fetal nucleated cells.
Dependencies are inferred from the claim text.