US 8,420,373 B2 · Permissive cells and uses thereof

Abstract

The invention relates generally to the field of virology. More particularly, the present invention relates to methods for determining the permissiveness of a cell for a virus that is a member of the family Arteriviridae or Coronaviridae or Asfarviridae, in particular for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). The invention further provides methods and compositions related to the generation of host cells permissive for a virus that is a member of the family Arteriviridae or Coronaviridae or Asfarviridae, in particular for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Methods of using said cells thus identified or thus generated, in preparing a culture of a virus that is a member of the family Arteriviridae or Coronaviridae or Asfarviridae, as well as the use of said virus for the purpose of vaccine production or diagnosis, are also provide by the present invention.

Claims14

Dependent claims

A method for preparing Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the method comprising:

determining CD163 expression in a cell line;
determining sialoadhesin expression in the cell line;
identifying a cell line having both CD163 and sialoadhesin expression as a permissive cell line for PRRSV;
infecting the identified cell line with PRRSV;
and
harvesting PRRSV from the cell line.

3 more elements

Dependencies are inferred from the claim text.

Permissive cells and uses thereof

Life of this patent

Permissive cells and uses thereof

Life of this patent

Abstract

Claims14