Potexvirus-derived replicon
AssigneeIcon Genetics GmbH
InventorsSylvestre Marillonnet, Carola Engler, Victor Klimyuk, Yuri Gleba
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Abstract
Nucleic acid comprising or encoding an RNA replica comprising, in this order, the following segments (i) to (iii): i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase or a function-conservative variant thereof; ii) a nucleic acid sequence comprising: a) a potexvirus triple gene block or a function-conservative variant thereof and b) a sequence encoding a potexviral coat protein or a function-conservative variant thereof; or a sequence encoding a tobago viral movement protein; and iii) a heterologous nucleic acid sequence expressible from said replica in a plant or in plant tissue.
Claims5
Nucleic acid comprising or encoding an RNA replicon comprising, in this order, the following segments (i) to (iii):
- (i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase;
- (ii) a nucleic acid sequence comprising:
- (a) a potexvirus triple gene block and
- (b) a sequence encoding a potexviral coat protein;
- wherein items (a) and (b) can be in any order in said nucleic acid sequence of item (ii) and
- (iii) a heterologous nucleic acid sequence comprising a coding sequence of a protein of interest and a sub-genomic promoter for expressing said protein of interest from said replicon in a plant or in plant tissue;
- wherein said potexvirus triple gene block encodes three proteins as follows:
- a first protein comprising the protein sequence of SEQ ID NO:
- 10;
- a second protein comprising the protein sequence of SEQ ID NO:
- 12;
- and a third protein comprising the protein sequence of SEQ ID NO:
- 14, wherein said potexviral coat protein comprises a protein sequence of SEQ ID NO:
- 8, and wherein said sequence of item (i) encodes a protein having a sequence identical to a protein encoded by SEQ ID NO:4.
A nucleic acid comprising a complementary sequence of a nucleic acid comprising or encoding an RNA replicon comprising, in this order, the following segments (i) to (iii):
- (i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase;
- (ii) a nucleic acid sequence comprising:
- (a) a potexvirus triple gene block and
- (b) a sequence encoding a potexviral coat protein;
- wherein items (a) and (b) can be in any order in said nucleic acid sequence of item (ii) and
- (iii) a heterologous nucleic acid sequence comprising a coding sequence of a protein of interest and a sub-genomic promoter for expressing said protein of interest from said replicon in a plant or in plant tissue;
- wherein said potexvirus triple gene block encodes three proteins as follows:
- a first protein comprising the protein sequence of SEQ ID NO:
- 10;
- a second protein comprising the protein sequence of SEQ ID NO:
- 12;
- and a third protein comprising the protein sequence of SEQ ID NO:
- 14, wherein said potexviral coat protein comprises a protein sequence of SEQ ID NO:
- 8, and wherein said sequence of item (i) encodes a protein having a sequence identical to a protein encoded by SEQ ID NO:4.
A process of expressing a heterologous nucleic acid sequence of interest in a plant or in plant tissue, comprising providing a plant or plant tissue with a nucleic acid comprising or encoding an RNA replicon comprising, in this order, the following segments (i) to (iii):
- (i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase;
- (ii) a nucleic acid sequence comprising:
- (a) a potexvirus triple gene block and
- (b) a sequence encoding a potexviral coat protein;
- wherein items (a) and (b) can be in any order in said nucleic acid sequence of item (ii) and
- (iii) a heterologous nucleic acid sequence comprising a coding sequence of a protein of interest and a sub-genomic promoter for expressing said protein of interest from said replicon in a plant or in plant tissue;
- wherein said potexvirus triple gene block encodes three proteins as follows:
- a first protein comprising the protein sequence of SEQ ID NO:
- 10;
- a second protein comprising the protein sequence of SEQ ID NO:
- 12;
- and a third protein comprising the protein sequence of SEQ ID NO:
- 14, wherein said potexviral coat protein comprises a protein sequence of SEQ ID NO:
- 8, and wherein said sequence of item (i) encodes a protein having a sequence identical to a protein encoded by SEQ ID NO:4.
Dependencies are inferred from the claim text.