Nucleic acid sequencing system and method
AssigneeIllumina, Inc.
InventorsRobert C. Kain, David L. Heiner, Chanfeng Zhao, Kevin Gunderson
Life of this patent
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Abstract
A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.
Claims20
A system for sequencing a plurality of nucleic acids, comprising:
- a fluidics handling system for facilitating assay reaction protocols;
- an imaging system for acquiring sequencing data from an array;
- diagnostic components configured to measure system parameters during operation of the sequencing system;
- quality control evaluation circuitry comprising a quality control routine configured to assess a quality of the sequencing system based upon a multiple step analysis;
- and
- control circuitry configured to alter operating conditions of the sequencing system based upon data collected by the diagnostic components or the quality evaluation circuitry.
A system for sequencing a plurality of nucleic acids, comprising:
- an imaging system for acquiring imaging data;
- diagnostic components configured to measure system parameters during operation of the sequencing system;
- quality evaluation circuitry configured to assess a decay in signal-to-noise ratio of the sequencing system based upon the measured system parameters;
- and
- control circuitry configured to alter operating conditions of the sequencing system based upon data collected by the diagnostic components or the quality evaluation circuitry.
A system for sequencing a plurality of nucleic acids, comprising:
- an imaging system for acquiring imaging data;
- quality evaluation circuitry configured to assess a decay in signal-to-noise ratio of the sequencing system based upon measured system parameters;
- and
- control circuitry configured to alter operating conditions of the sequencing system based upon data collected by the quality evaluation circuitry.
Dependencies are inferred from the claim text.